The polyene antifungal candicidin is selectively packaged into membrane vesicles in Streptomyces S4

In recent years, much attention has been focused on the biogenesis, engineering and utilisation of outer membrane vesicles (OMVs) in Gram-negative bacteria in a range of environments and niches. While the precise mechanism of biogenesis is unknown, it is focused on the modification of the Gram-negat...

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Bibliographic Details
Published in:Archives of microbiology 2022-05, Vol.204 (5), p.289-289, Article 289
Main Authors: Blackburn, Sarah A., Shepherd, Mark, Robinson, Gary K.
Format: Article
Language:English
Subjects:
Animals
Antifungal agents
Antifungal Agents - metabolism
Antifungal Agents - pharmacology
Ants - microbiology
Bacteria
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Candicidin
Candicidin - metabolism
Cell Biology
Cell walls
Ecology
Fungicides
Gram-negative bacteria
Life Sciences
Membrane vesicles
Membranes
Metabolites
Microbial Ecology
Microbiology
Original Paper
Peptidoglycans
Periplasm
Polyenes - metabolism
Polyenes - pharmacology
Secondary metabolites
Streptomyces - genetics
Streptomyces - metabolism
Vesicles
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Summary:In recent years, much attention has been focused on the biogenesis, engineering and utilisation of outer membrane vesicles (OMVs) in Gram-negative bacteria in a range of environments and niches. While the precise mechanism of biogenesis is unknown, it is focused on the modification of the Gram-negative cell wall to facilitate blebbing at sites of weakness in and around the characteristically thin peptidoglycan layer within the periplasm. Here, we investigate the biogenesis of membrane vesicles (MVs) in the Gram-positive organism Streptomyces albus S4 (Seipke et al. J Bacteriol 193:4270–4271, 2011 and Fazal et al. Antonie Van Leeuwenhoek 113:511–520, 2020). The S. albus S4 strain is an antifungal (candicidin and antimycin) producing organism that was isolated from attine ants (Barke et al. BMC Biol 8:109, 2010). The biogenesis and characterisation of S. albus S4 MVs is demonstrated using the wild-type (WT) and mutant strains ΔantC (no antimycin production) ΔfscC (no candicidin production) and ΔantC Δ fscC (produces neither antimycin nor candicidin). Here, we have shown that the S. albus S4 strain produces MVs and that these are comprised of both specific protein profiles and secondary metabolites, with a clear demonstration of the ability to selectively package one antifungal (candicidin) but not the other (antimycin).
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-02906-w