CdTe QD-based inhibition and reactivation assay of acetylcholinesterase for the detection of organophosphorus pesticides

An enzyme immobilized glutathione (GSH)-capped CdTe quantum dot (QD)-based fluorescence assay has been developed for monitoring organophosphate pesticides. In principle, GSH-capped CdTe QDs exhibit higher sensitivity towards H 2 O 2 produced from the active enzymatic reaction of acetylcholinesterase...

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Bibliographic Details
Published in:RSC advances 2020-06, Vol.1 (41), p.2419-2422
Main Authors: Korram, Jyoti, Dewangan, Lakshita, Karbhal, Indrapal, Nagwanshi, Rekha, Vaishanav, Sandeep K, Ghosh, Kallol K, Satnami, Manmohan L
Format: Article
Language:English
Subjects:
Activation
Assaying
Biosensors
Cadmium tellurides
Chains
Chemistry
Choline
Fluorescence
Glutathione
Hydrogen peroxide
Optical properties
Organophosphates
Oximes
Pesticides
Quantum dots
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Summary:An enzyme immobilized glutathione (GSH)-capped CdTe quantum dot (QD)-based fluorescence assay has been developed for monitoring organophosphate pesticides. In principle, GSH-capped CdTe QDs exhibit higher sensitivity towards H 2 O 2 produced from the active enzymatic reaction of acetylcholinesterase (AChE) and choline oxidase (CHOx), which results in the fluorescence (FL) "turn-off" of the GSH-capped CdTe QDs. A "turn-on" FL of the CdTe QDs at 520 nm was recovered in the presence of organophosphate (OP). The FL changes of the GSH-capped CdTe QD/AChE/CHOx biosensor reasonably correspond to the amount of OP pesticides. The detection limit of the CdTe/AChE/CHOx biosensor towards paraoxon, dichlorvos, malathion and triazophos was 1.62 × 10 −15 M, 75.3 × 10 −15 M, 0.23 × 10 −9 M and 10.6 × 10 −12 M, respectively. The GSH-capped CdTe QDs/AChE/CHOx biosensor was applied as a FL nanoprobe for assaying the enzymatic activity of AChE. The inhibited AChE was reactivated up to 94% using pyridine oximate (2-PyOx − ), and functionalized pyridinium oximates (4-C 12 PyOx − and 4-C 18 PyOx − ) of varying chain lengths. It was found that the reactivation potency of the tested oximes varied with the chain length of the oximes. This biosensing system offers the promising benefit for the determination of the OP pesticides in food, water and environmental samples. An enzyme immobilized glutathione (GSH)-capped CdTe quantum dot (QD)-based fluorescence assay has been developed for monitoring organophosphate pesticides.
ISSN:2046-2069
2046-2069
DOI:10.1039/d0ra03055d