“X” marks the spot: Mining the gold in CasX for gene editing

In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved th...

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Bibliographic Details
Published in:Molecular cell 2022-03, Vol.82 (6), p.1083-1085
Main Authors: Roth, Mitchell O., Li, Hong
Format: Article
Language:English
Subjects:
Clustered Regularly Interspaced Short Palindromic Repeats
CRISPR-Cas Systems
Gene Editing
Gold
Humans
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Summary:In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10–20 fold in vitro and mean-editing efficiency by ∼2–3 fold in human cells. In this issue of Molecular Cell, Tsuchida et al. (2022) present a successful structure-guided effort in improving genome-editing efficiencies of CRISPR-CasX from Deltaproteobacteria (DpbCasX) and Planctomycetes (PlmCasX). Engineered variants that stabilize the active conformational state improved the catalytic efficiency by ∼10–20 fold in vitro and mean-editing efficiency by ∼2–3 fold in human cells.
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2022.02.024