Yunnanterpene G, a spiro-triterpene from the roots of Cimicifuga foetida , downregulates the expression of CD147 and MMPs in PMA differentiated THP-1 cells

A new cycloartane triterpene, yunnanterpene G (1), containing an oxaspiro[5.4]decane moiety, was purified from the roots of . The new structure was determined from spectroscopic data and the X-ray diffraction method. Biological evaluations revealed that compound 1 significantly inhibited the mRNA ex...

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Bibliographic Details
Published in:RSC advances 2018-01, Vol.8 (27), p.15036-15043
Main Authors: Lu, Ni-Hong, Zhang, Zi-Wei, Guo, Rui-Wei, Yang, Li-Xia, Song, Ya-Xian, Ye, Jin-Shan, Shi, Yan-Kun
Format: Article
Language:English
Subjects:
Atherosclerosis
Chemistry
Gene expression
Phosphorylation
Roots
X-ray diffraction
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Summary:A new cycloartane triterpene, yunnanterpene G (1), containing an oxaspiro[5.4]decane moiety, was purified from the roots of . The new structure was determined from spectroscopic data and the X-ray diffraction method. Biological evaluations revealed that compound 1 significantly inhibited the mRNA expression of the atherosclerosis-related adhesion molecule CD147 (extracellular matrix metalloproteinase inducer, EMMPRIN), and proteolytic enzymes matrix metalloproteinase 2 (MMP-2), MMP-9 and MMP-14, in a dose-dependent manner in phorbol-12-myristate-13-acetate-induced human monocytic THP-1 cells by quantitative real-time PCR method. At the same time, the migration ability of the induced THP-1 cells was potently inhibited. Furthermore, western blot experiments showed that compound 1 at 25 μM strongly suppressed phosphorylation of NF-κB p65 and p38 MAPK in the differentiated THP-1 cells.
ISSN:2046-2069
2046-2069
DOI:10.1039/c8ra01895b