Establishment of Patient-derived Orthotopic Xenografts (PDX) as Models for Pancreatic Ductal Adenocarcinoma

Pancreatic cancer (PC) is one of the leading causes of cancer-related death. The purpose of the present study was to establish a patient-derived orthotopic xenograft model (PDOX) for pancreatic ductal adenocarcinoma (PDAC), thus providing a tumor microenvironment resembling that of the human pancrea...

Full description

Saved in:
Bibliographic Details
Published in:In vivo (Athens) 2022-05, Vol.36 (3), p.1114-1119
Main Authors: Magouliotis, Dimitrios E, Lafazanis, Kostas, Koutsougianni, Fani, Sakellaridis, Nikos, Ioannou, Maria, Zacharoulis, Dimitris, Dimas, Konstantinos
Format: Article
Language:English
Subjects:
Animals
Carcinoma, Pancreatic Ductal - pathology
Disease Models, Animal
Heterografts
Humans
Mice
Mice, Inbred NOD
Mice, SCID
Pancreatic Neoplasms
Pancreatic Neoplasms - pathology
Tumor Microenvironment
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pancreatic cancer (PC) is one of the leading causes of cancer-related death. The purpose of the present study was to establish a patient-derived orthotopic xenograft model (PDOX) for pancreatic ductal adenocarcinoma (PDAC), thus providing a tumor microenvironment resembling that of the human pancreas to identify novel potential biomarkers and treatment regimens. PDAC tissue samples were received from 35 patients, following informed consent, and three mouse strains were implemented. Successful PDOX engraftment was performed in nonobese diabetic/severe combined immunodeficient (NOD/SCID) and NOD/SCID gamma (NSG) mice. Nonetheless, we found a higher rate of successful engraftment and tumor growth in NSG compared to NOD/SCID mice, possibly owning to the different level of immunosuppression and more specifically of the natural killer cells presence. Our suggested PDOX model represents a preclinical cancer research model with a high affinity for the patient's tumor microenvironment, thus enabling the acceleration of PDAC research.
ISSN:0258-851X
1791-7549
DOI:10.21873/invivo.12809