Among Antibody-Like Molecules, Monobodies, Able to Interact with Nucleocapsid Protein of SARS-CoV Virus, There Are Monobodies with High Affinity to Nucleocapsid Protein of SARS-CoV-2 Virus

Seven amino acid sequences of antibody mimetics molecules, monobodies, capable of interacting with the nucleocapsid protein of the SARS-CoV virus, were taken from the literature. Nucleotide sequences of monobody genes were obtained by gene synthesis, which were expressed in E. coli and isolated usin...

Full description

Saved in:
Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2022-04, Vol.503 (1), p.90-92
Main Authors: Khramtsov, Y. V., Ulasov, A. V., Lupanova, T. N., Georgiev, G. P., Sobolev, A. S.
Format: Article
Language:English
Subjects:
Affinity
Antibodies
Biochemistry
Biochemistry, Biophysics, and Molecular Biology
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Life Sciences
Molecular Biology
Nucleocapsids
Proteins
Severe acute respiratory syndrome coronavirus 2
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Seven amino acid sequences of antibody mimetics molecules, monobodies, capable of interacting with the nucleocapsid protein of the SARS-CoV virus, were taken from the literature. Nucleotide sequences of monobody genes were obtained by gene synthesis, which were expressed in E. coli and isolated using Ni-NTA chromatography. It was shown by thermophoresis that three of the seven selected antibody-like molecules can interact with high affinity (dissociation constant of tens of nM) with the nucleocapsid protein of the SARS-CoV-2 virus. For the remaining four monobodies, only low affinity binding with a dissociation constant of several μM was found.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672922020077