Passion fruit seed extract protects beta‐amyloid‐induced neuronal cell death in a differentiated human neuroblastoma SH‐SY5Y cell model

• Published in: Food science & nutrition 2022-05, Vol.10 (5), p.1461-1468
• Main Authors: Sato, Akira, Tagai, Nozomi, Ogino, Yoko, Uozumi, Haruka, Kawakami, Shinpei, Yamamoto, Takayuki, Tanuma, Sei‐ichi, Maruki‐Uchida, Hiroko, Mori, Sadao, Morita, Minoru
• Format: Article
• Language: English
• Subjects: Acids; Alzheimer's disease; Apoptosis; Brain; Brain-derived neurotrophic factor; Cancer; Cell culture; Cell death; Cell differentiation; Fragmentation; Fruits; functional food; Functional foods & nutraceuticals; Glucose; Morphology; Mortality; neurite fragmentation; Neuroblastoma; Neuroblastoma cells; Neurodegenerative diseases; neuronal cell death; Neuroprotection; Neurotoxicity; Original; Original Research; Passion fruit; passion fruit seed extract; Peptides; Piceatannol; Polyphenols; Proteins; Statistical analysis; Stilbene
• ISSN: 2048-7177; 2048-7177
• DOI: 10.1002/fsn3.2757

Alzheimer's disease (AD) is a progressive neurodegenerative disease with accompanying perceptive disorder. We previously reported that decreasing levels of brain‐derived neurotrophic factor (BDNF) promoted beta‐amyloid (Aβ)‐induced neuronal cell death in neuron‐like differentiated SH‐SY5Y (ndSH‐SY5Y) human neuroblastoma cells in an AD mimic cell model. We investigated the neuroprotective effects of passion fruit seed extract (PFSE) and one of the main stilbene compounds, piceatannol, in an AD cell model using ndSH‐SY5Y cells. Both PFSE and piceatannol were found to protect Aβ‐induced neurite fragmentation in the cell model (protection efficacy; 34% in PFSE and 36% in piceatannol). In addition, both PFSE and piceatannol suppress Aβ‐induced neuronal cell death in the cell model (inhibitory effect; 27% in PFSE and 32% in piceatannol). Our study is the first to report that piceatannol‐rich PFSE can repress Aβ‐induced neuronal cell death by protecting against neurite fragmentation in the AD human cell model. These findings suggest that piceatannol‐rich PFSE can be considered a potentially neuroprotective functional food for both prevention and treatment of AD. We investigated the neuroprotective effect of piceatannol and passion fruit seed extract (PFSE) rich in this compound in an Alzheimer's disease (AD) mimic cell model. Both piceatannol and PFSE were found to protect beta‐amyloid‐induced neurite fragmentation in the AD cell model. Both piceatannol and PFSE were proven to suppress beta‐amyloid‐induced neuronal cell death in the AD cell model.