Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging

Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effects of the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adver...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2022, Vol.84(4), pp.585-592
Main Authors: NISHI, Misaki, SUGIO, Shouta, HIRANO, Tetsushi, KATO, Daisuke, WAKE, Hiroaki, SHODA, Asuka, MURATA, Midori, IKENAKA, Yoshinori, TABUCHI, Yoshiaki, MANTANI, Youhei, YOKOYAMA, Toshifumi, HOSHI, Nobuhiko
Format: Article
Language:English
Subjects:
Animals
Calcium imaging
Calcium influx
clothianidin
Cortex (somatosensory)
DNA microarrays
Gene expression
Guanidines
Homeostasis
Inflammation
Insecticides - toxicity
Mammals
Mice
Microscopy
Microscopy - veterinary
neonicotinoid
Neonicotinoids - toxicity
Neuroimaging
Neurons
neurotransmission
No-Observed-Adverse-Effect Level
Pesticides
Thiazoles
Toxicology
two-photon calcium imaging
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Summary:Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effects of the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.22-0013