Chondroitin sulfate E downregulates N-cadherin and suppresses myotube formation

Myogenesis, the formation of muscle fibers, is affected by certain glycoproteins, including chondroitin sulfate (CS), which are involved in various cellular processes. We aimed to investigate the mechanism underlying CS-E-induced suppression of myotube formation using the myoblast cell line C2C12. D...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2022, Vol.84(4), pp.494-501
Main Authors: SATOH, Fumi, SUGIURA, Akihiro, TASHIRO, Jiro, HOSAKA, Yoshinao Z., WARITA, Katsuhiko
Format: Article
Language:English
Subjects:
Actin
Animals
Biochemistry
Cadherins - metabolism
Cell Differentiation
Cell Fusion - veterinary
Chondroitin sulfate
chondroitin sulfate E
Chondroitin Sulfates - metabolism
Chondroitin Sulfates - pharmacology
Cofilin
Cytoskeleton
Gene expression
Glycoproteins
Muscle Development
Muscle Fibers, Skeletal
myoblast
Myoblasts
Myogenesis
Myosin
myotube
Myotubes
N-Cadherin
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Summary:Myogenesis, the formation of muscle fibers, is affected by certain glycoproteins, including chondroitin sulfate (CS), which are involved in various cellular processes. We aimed to investigate the mechanism underlying CS-E-induced suppression of myotube formation using the myoblast cell line C2C12. Differentiated cells treated with 0.1 mg/ml CS-E for nine days showed multinucleated and rounded myotubes with myosin heavy chain positivity. No difference was found between the CS-E-treated group with rounded myotubes and CS (−) controls with elongated myotubes in the levels of phospho-cofilin, a protein involved in the dynamics of actin cytoskeleton. Interestingly, N-cadherin, which is involved in the gene expression of myoblast fusion factors (myomaker and myomixer), was significantly downregulated at both the mRNA and protein levels following CS-E treatment. These results suggest that N-cadherin downregulation is one of the mechanisms underlying the CS-E-induced suppression of myotube formation.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.21-0662