Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR

A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtaine...

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Bibliographic Details
Published in:Applied and environmental microbiology 1999, Vol.65 (1), p.287-290
Main Authors: Kim, S.H, Uzunovic, A, Breuil, C
Format: Article
Language:English
Subjects:
Ascomycota - genetics
Ascomycota - isolation & purification
Base Sequence
Biological and medical sciences
Biotechnology
Deoxyribonucleic acid
DNA
DNA Primers - genetics
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
Fundamental and applied biological sciences. Psychology
Fungi
genbank/af081129
genbank/af081130
genbank/af081131
genbank/af081132
genbank/af081133
genbank/af081134
Industrial applications and implications. Economical aspects
internal transcribed spacers
Methods
Microbiology
Molecular Sequence Data
Mycological methods and techniques used in mycology
Mycology
nucleotide sequences
Ophiostoma piceae
Other applications
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - statistics & numerical data
ribosomal DNA
Sensitivity and Specificity
stain fungi
Staining and Labeling
Wood
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Summary:A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.65.1.287-290.1999