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Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtaine...
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| Published in: | Applied and environmental microbiology 1999, Vol.65 (1), p.287-290 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c515t-2e380adfce3045c4459e81694c5289280837a690b648d354e5fe3c0d8693896b3 |
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| cites | cdi_FETCH-LOGICAL-c515t-2e380adfce3045c4459e81694c5289280837a690b648d354e5fe3c0d8693896b3 |
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| container_issue | 1 |
| container_start_page | 287 |
| container_title | Applied and environmental microbiology |
| container_volume | 65 |
| creator | Kim, S.H Uzunovic, A Breuil, C |
| description | A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi. |
| doi_str_mv | 10.1128/aem.65.1.287-290.1999 |
| format | article |
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By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.65.1.287-290.1999</identifier><identifier>PMID: 9872792</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Ascomycota - genetics ; Ascomycota - isolation & purification ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Deoxyribonucleic acid ; DNA ; DNA Primers - genetics ; DNA, Fungal - genetics ; DNA, Fungal - isolation & purification ; Fundamental and applied biological sciences. 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Economical aspects ; internal transcribed spacers ; Methods ; Microbiology ; Molecular Sequence Data ; Mycological methods and techniques used in mycology ; Mycology ; nucleotide sequences ; Ophiostoma piceae ; Other applications ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - statistics & numerical data ; ribosomal DNA ; Sensitivity and Specificity ; stain fungi ; Staining and Labeling ; Wood</subject><ispartof>Applied and environmental microbiology, 1999, Vol.65 (1), p.287-290</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Jan 1999</rights><rights>Copyright © 1999, American Society for Microbiology 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-2e380adfce3045c4459e81694c5289280837a690b648d354e5fe3c0d8693896b3</citedby><cites>FETCH-LOGICAL-c515t-2e380adfce3045c4459e81694c5289280837a690b648d354e5fe3c0d8693896b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC91015/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC91015/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1695487$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9872792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, S.H</creatorcontrib><creatorcontrib>Uzunovic, A</creatorcontrib><creatorcontrib>Breuil, C</creatorcontrib><title>Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. 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Economical aspects</subject><subject>internal transcribed spacers</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>nucleotide sequences</subject><subject>Ophiostoma piceae</subject><subject>Other applications</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - statistics & numerical data</subject><subject>ribosomal DNA</subject><subject>Sensitivity and Specificity</subject><subject>stain fungi</subject><subject>Staining and Labeling</subject><subject>Wood</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkV9vFCEUxYnR1LX6ERqJMb7NeIGBgcSXZlP_pc2aap8JyzAtzQxMYUbTby_NbtraF59IOL9zuYeD0BGBmhAqPxo31oLXpKayragqt0qpZ2hFQMmKMyaeoxWAUhWlDbxEr3K-BoAGhDxAB0q2tFV0hb6fm8l3uHOzs7OPAcceb6YrH_McR4Mnb51x2IQOb2p8s7hkl4x9wHk2PrgO_4mxw9tb_GN9_hq96M2Q3Zv9eYguPp_8Wn-tTjdfvq2PTyvLCZ8r6pgE0_XWMWi4bRqunCRCNZZTqagEyVojFGxFIzvGG8d7xyx0UigmldiyQ_RpN3datqPrrAtzMoOekh9NutXReP2vEvyVvoy_tSJAeLF_2NtTLIHyrEefrRsGE1xcshaKsxYY_S9IWiIZEayA756A13FJofyBpsAVpyBlgfgOsinmnFx_vzABfVeoPj4504JrokuhuhSq7wotvqPHae9d-waL_n6vm2zN0CcTrM8Pw0ucRrYFe7vDehO1uUwFufhJgTAoLxEuOPsLF6Cwsw</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Kim, S.H</creator><creator>Uzunovic, A</creator><creator>Breuil, C</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1999</creationdate><title>Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR</title><author>Kim, S.H ; Uzunovic, A ; Breuil, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-2e380adfce3045c4459e81694c5289280837a690b648d354e5fe3c0d8693896b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Ascomycota - genetics</topic><topic>Ascomycota - isolation & purification</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Primers - genetics</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Fungal - isolation & purification</topic><topic>Fundamental and applied biological sciences. 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Economical aspects</topic><topic>internal transcribed spacers</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>nucleotide sequences</topic><topic>Ophiostoma piceae</topic><topic>Other applications</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - statistics & numerical data</topic><topic>ribosomal DNA</topic><topic>Sensitivity and Specificity</topic><topic>stain fungi</topic><topic>Staining and Labeling</topic><topic>Wood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, S.H</creatorcontrib><creatorcontrib>Uzunovic, A</creatorcontrib><creatorcontrib>Breuil, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, S.H</au><au>Uzunovic, A</au><au>Breuil, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1999</date><risdate>1999</risdate><volume>65</volume><issue>1</issue><spage>287</spage><epage>290</epage><pages>287-290</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9872792</pmid><doi>10.1128/aem.65.1.287-290.1999</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0099-2240 |
| ispartof | Applied and environmental microbiology, 1999, Vol.65 (1), p.287-290 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_91015 |
| source | Open Access: PubMed Central; ASM_美国微生物学会期刊 |
| subjects | Ascomycota - genetics Ascomycota - isolation & purification Base Sequence Biological and medical sciences Biotechnology Deoxyribonucleic acid DNA DNA Primers - genetics DNA, Fungal - genetics DNA, Fungal - isolation & purification Fundamental and applied biological sciences. Psychology Fungi genbank/af081129 genbank/af081130 genbank/af081131 genbank/af081132 genbank/af081133 genbank/af081134 Industrial applications and implications. Economical aspects internal transcribed spacers Methods Microbiology Molecular Sequence Data Mycological methods and techniques used in mycology Mycology nucleotide sequences Ophiostoma piceae Other applications polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data ribosomal DNA Sensitivity and Specificity stain fungi Staining and Labeling Wood |
| title | Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR |
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