microRNA‐126 inhibits vascular cell adhesion molecule‐1 and interleukin‐1beta in human dental pulp cells

Background Vascular cell adhesion molecule (VCAM‐1) mediates pulpitis via regulating interleukin (IL)‐1β. microRNA (miR)‐126 was reported to regulate the VCAM‐1 under many different pathophysiological circumstances. We investigated variations of miR‐126 and VCAM‐1 in inflamed patient pulp tissues an...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2022-05, Vol.36 (5), p.e24371-n/a
Main Authors: Jiang, Long, Krongbaramee, Tadkamol, Lin, Xinhai, Zhu, Min, Zhu, Yaqin, Hong, Liu
Format: Article
Language:English
Subjects:
Adhesives
Angiogenesis
Blood vessels
Cell adhesion & migration
Cell adhesion molecules
Dental pulp
human dental pulp cells
Infections
Inflammation
interleukin‐1β
Kinases
Lipopolysaccharides
MicroRNAs
microRNA‐126
miRNA
Nanoparticles
Polyethyleneimine
Statistical analysis
Teeth
Variance analysis
vascular cell adhesion molecule‐1
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Summary:Background Vascular cell adhesion molecule (VCAM‐1) mediates pulpitis via regulating interleukin (IL)‐1β. microRNA (miR)‐126 was reported to regulate the VCAM‐1 under many different pathophysiological circumstances. We investigated variations of miR‐126 and VCAM‐1 in inflamed patient pulp tissues and determined potential roles of miR‐126 in pulpitis using human dental pulp cells (hDPCs) in vitro. Methods We quantitatively measured the transcripts of miR‐126 and VCAM‐1 in inflamed human pulp tissues using qRT‐PCR and compared with those from healthy human pulp tissues. In addition, we transfected miR‐126 in hDPCs using plasmid DNA (pDNA)‐encoding miR‐126 delivered by polyethylenimine (PEI) nanoparticles. Results The irreversible pulpitis significantly reduced miR‐126 and increased the transcript of VCAM‐1 in pulp tissues (p 
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.24371