Interplay of Affinity and Surface Tethering in Protein Recognition

Surface-tethered ligand–receptor complexes are key components in biological signaling and adhesion. They also find increasing utility in single-molecule assays and biotechnological applications. Here, we study the real-time binding kinetics between various surface-immobilized peptide ligands and the...

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Bibliographic Details
Published in:The journal of physical chemistry letters 2022-05, Vol.13 (18), p.4021-4028
Main Authors: Imran, Ali, Moyer, Brandon S., Wolfe, Aaron J., Cosgrove, Michael S., Makarov, Dmitrii E., Movileanu, Liviu
Format: Article
Language:English
Subjects:
Intrinsically Disordered Proteins
Kinetics
Letter
Ligands
Peptides
Physical Insights into Chemistry, Catalysis, and Interfaces
Protein Binding
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Summary:Surface-tethered ligand–receptor complexes are key components in biological signaling and adhesion. They also find increasing utility in single-molecule assays and biotechnological applications. Here, we study the real-time binding kinetics between various surface-immobilized peptide ligands and their unrestrained receptors. A long peptide tether increases the association of ligand–receptor complexes, experimentally proving the fly casting mechanism where the disorder accelerates protein recognition. On the other hand, a short peptide tether enhances the complex dissociation. Notably, the rate constants measured for the same receptor, but under different spatial constraints, are strongly correlated to one another. Furthermore, this correlation can be used to predict how surface tethering on a ligand–receptor complex alters its binding kinetics. Our results have immediate implications in the broad areas of biomolecular recognition, intrinsically disordered proteins, and biosensor technology.
ISSN:1948-7185
1948-7185
DOI:10.1021/acs.jpclett.2c00621