Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

• Published in: Biotechnology for biofuels 2022-05, Vol.15 (1), p.50-50, Article 50
• Main Authors: Yuan, Huiling, Zhou, Ying, Lin, Yuping, Tu, Ran, Guo, Yufeng, Zhang, Yuanyuan, Wang, Qinhong
• Format: Article
• Language: English
• Subjects: Ascomycota; Cellulase; Chemical properties; Chromatin remodeling; Directed evolution; Droplets; Enzymes; Evolution; Fungi; Gene deletion; Gene mutations; Genetic aspects; Genomes; Genomics; High-throughput screening; Impact factors; Methods; Microbial enzymes; Microbiological research; Microbiological synthesis; Microfluidics; Microorganisms; Microscopy; Mutagenesis; Mutants; Mutation; Performance evaluation; Physiological aspects; Pichia pastoris; Point mutation; Production processes; Protein expression; Proteins; Room temperature; Screening; Yeast
• ISSN: 2731-3654; 2731-3654; 1754-6834
• DOI: 10.1186/s13068-022-02150-w

Pichia pastoris is a widely used host organism for heterologous production of industrial proteins, such as cellulases. Although great progress has been achieved in improving protein expression in P. pastoris, the potential of the P. pastoris expression system has not been fully explored due to unknown genomic impact factors. Recently, whole-cell directed evolution, employing iterative rounds of genome-wide diversity generation and high-throughput screening (HTS), has been considered to be a promising strategy in strain improvement at the genome level. In this study, whole-cell directed evolution of P. pastoris, employing atmospheric and room temperature plasma (ARTP) mutagenesis and droplet-based microfluidic HTS, was developed to improve heterogenous cellulase production. The droplet-based microfluidic platform based on a cellulase-catalyzed reaction of releasing fluorescence was established to be suitable for methanol-grown P. pastoris. The validation experiment showed a positive sorting efficiency of 94.4% at a sorting rate of 300 droplets per second. After five rounds of iterative ARTP mutagenesis and microfluidic screening, the best mutant strain was obtained and exhibited the cellulase activity of 11,110 ± 523 U/mL, an approximately twofold increase compared to the starting strain. Whole-genome resequencing analysis further uncovered three accumulated genomic alterations in coding region. The effects of point mutations and mutant genes on cellulase production were verified using reconstruction of point mutations and gene deletions. Intriguingly, the point mutation Rsc1 was observed in all the top-performing producers selected from each round, and gene deletion analysis confirmed that Rsc1, a component of the RSC chromatin remodeling complex, might play an important role in cellulase production. We established a droplet-based microfluidic HTS system, thereby facilitating whole-cell directed evolution of P. pastoris for enhancing cellulase production, and meanwhile identified genomic alterations by whole-genome resequencing and genetic validation. Our approaches and findings would provide guides to accelerate whole-cell directed evolution of host strains and enzymes of high industrial interest.