Fine-tuning the transcriptional regulatory model of adaptation response to phosphate stress in maize (Zea mays L.)

• Published in: Physiology and molecular biology of plants 2022-04, Vol.28 (4), p.885-898
• Main Authors: Yadava, Pranjal, Dayaman, Vikram, Agarwal, Astha, Kumar, Krishan, Singh, Ishwar, Verma, Rachana, Kaul, Tanushri
• Format: Article
• Language: English
• Subjects: Acid phosphatase; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Biology; Cereals; Corn; Deprivation; Fertilizer application; Fertilizers; Gene regulation; Genes; Genotypes; Green revolution; Homeostasis; Inbreeding; Leaves; Life Sciences; Molecular modelling; Phosphorus; Plant Physiology; Plant Sciences; Plant tissues; Research Article; Ribonuclease; Root hairs; Roots; Transcription; Zea mays
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-022-01155-x

The post green revolution agriculture is based on generous application of fertilizers and high-yielding genotypes that are suited for such high input regimes. Cereals, like maize ( Zea mays L.) are capable of utilizing less than 20% of the applied inorganic phosphate (Pi) - a non-renewable fertilizer resource. A greater understanding of the molecular mechanisms underlying the acquisition, transportation and utilization of Pi may lead to engineering genotypes with high phosphorus use efficiency. In this study, we carried out functional domain similarity analysis, promoter analysis and comparative transcriptional expression profiling of 12 selected Pi responsive genes in the Pi stress tolerant maize inbred line HKI-163 under sufficient and deficient Pi conditions. Pi starvation led to significant increase in root length; marked proliferation of root hairs and lesser number of crown roots. Eleven genes were significantly up or down regulated in Pi deficient condition. The putative acid phosphatase, ZmACP5 expression was up regulated by 162.81 and 74.40 fold in root and leaf tissues, respectively. The RNase, ZmRNS1 showed 115 fold up regulation in roots under Pi deprivation. Among the two putative high affinity Pi transporters ZmPht1;4 was found specific to root, whereas ZmPht2 was found to be up regulated in both root and leaf tissues. The genes involved in Pi homeostasis pathway ( ZmSIZ1, SPX1 and Pho2 ) were up regulated in root and leaf. In light of the expression profiling of selected regulatory genes, an updated model of transcriptional regulation under Pi starvation in maize has been presented.