Maize brace root mechanics vary by whorl, genotype and reproductive stage

• Published in: Annals of botany 2022-05, Vol.129 (6), p.657-668
• Main Authors: Hostetler, Ashley N, Erndwein, Lindsay, Ganji, Elahe, Reneau, Jonathan W, Killian, Megan L, Sparks, Erin E
• Format: Article
• Language: English
• Subjects: Genotype; Original; Plant Roots - genetics; Reproduction; Zea mays - genetics
• ISSN: 0305-7364; 1095-8290
• DOI: 10.1093/aob/mcac029

Root lodging is responsible for significant crop losses worldwide. During root lodging, roots fail by breaking, buckling or pulling out of the ground. In maize, above-ground roots, called brace roots, have been shown to reduce susceptibility to root lodging. However, the underlying structural-functional properties of brace roots that prevent root lodging are poorly defined. In this study, we quantified structural mechanical properties, geometry and bending moduli for brace roots from different whorls, genotypes and reproductive stages. Using 3-point bend tests, we show that brace root mechanics are variable by whorl, genotype and reproductive stage. Generally, we find that within each genotype and reproductive stage, the brace roots from the first whorl (closest to the ground) had higher structural mechanical properties and a lower bending modulus than brace roots from the second whorl. There was additional variation between genotypes and reproductive stages. Specifically, genotypes with higher structural mechanical properties also had a higher bending modulus, and senesced brace roots had lower structural mechanical properties than hydrated brace roots. Collectively these results highlight the importance of considering whorl-of-origin, genotype and reproductive stage for the quantification of brace root mechanics, which is important for mitigating crop loss due to root mechanical failure.