Inter‐species lateral gene transfer focused on the Chlamydia plasticity zone identifies loci associated with immediate cytotoxicity and inclusion stability

• Published in: Molecular microbiology 2021-12, Vol.116 (6), p.1433-1448
• Main Authors: Dimond, Zoe E., Suchland, Robert J., Baid, Srishti, LaBrie, Scott D., Soules, Katelyn R., Stanley, Jacob, Carrell, Steven, Kwong, Forrest, Wang, Yibing, Rockey, Daniel D., Hybiske, Kevin, Hefty, P. Scott
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Chimeras; Chlamydia; Chlamydia Infections - microbiology; Chlamydia muridarum - genetics; Chlamydia muridarum - metabolism; Chlamydia trachomatis - genetics; Chlamydia trachomatis - metabolism; cytopathic effect; Cytotoxicity; Cytotoxins; Divergence; Female; Gene transfer; Gene Transfer, Horizontal; Genetic Variation; Genital tract; Genomics; Homology; Host specificity; Humans; Infections; Mice; Mice, Inbred C57BL; Phenotypes; Plastic properties; Plasticity; plasticity zone; Sexually transmitted diseases; STD; Toxicity; Tropism
• ISSN: 0950-382X; 1365-2958; 1365-2958
• DOI: 10.1111/mmi.14832

Chlamydia muridarum actively grows in murine mucosae and is a representative model of human chlamydial genital tract disease. In contrast, C. trachomatis infections in mice are limited and rarely cause disease. The factors that contribute to these differences in host adaptation and specificity remain elusive. Overall genomic similarity leads to challenges in the understanding of these significant differences in tropism. A region of major genetic divergence termed the plasticity zone (PZ) has been hypothesized to contribute to the host specificity. To evaluate this hypothesis, lateral gene transfer was used to generate multiple hetero‐genomic strains that are predominately C. trachomatis but have replaced regions of the PZ with those from C. muridarum. In vitro analysis of these chimeras revealed C. trachomatis‐like growth as well as poor mouse infection capabilities. Growth‐independent cytotoxicity phenotypes have been ascribed to three large putative cytotoxins (LCT) encoded in the C. muridarum PZ. However, analysis of PZ chimeras supported that gene products other than the LCTs are responsible for cytopathic and cytotoxic phenotypes. Growth analysis of associated chimeras also led to the discovery of an inclusion protein, CTL0402 (CT147), and homolog TC0424, which was critical for the integrity of the inclusion and preventing apoptosis. The major region of genomic diversity between the C. trachomatis and C. muridarum is the 25–50 Kb plasticity zone. Replacement of the 50 Kb PZ from C. muridarum into C. trachomatis did not enhance infection capabilities in mice. Generation of these chimera strains enabled the discovery of an inclusion membrane protein, CT147, and importance to maintaining membrane integrity.