Ultrastable Silver Nanoparticles for Rapid Serology Detection of Anti-SARS-CoV‑2 Immunoglobulins G

Dipstick assays using silver nanoparticles (AgNPs) stabilized by a thin calix[4]­arene-based coating were developed and used for the detection of Anti-SARS-CoV-2 IgG in clinical samples. The calixarene-based coating enabled the covalent bioconjugation of the SARS-CoV-2 Spike Protein via the classica...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-05, Vol.94 (20), p.7383-7390
Main Authors: Gosselin, Bryan, Retout, Maurice, Dutour, Raphaël, Troian-Gautier, Ludovic, Bevernaegie, Robin, Herens, Sophie, Lefèvre, Philippe, Denis, Olivier, Bruylants, Gilles, Jabin, Ivan
Format: Article
Language:English
Subjects:
Antibodies, Viral
Assaying
Calixarenes
Chemistry
Coatings
COVID-19 - diagnosis
Gold
Humans
Immunoassay - methods
Immunoassays
Immunoglobulin G
Immunoglobulins
Metal Nanoparticles
Nanoparticles
SARS-CoV-2
Sensitivity
Sensitivity and Specificity
Serology
Severe acute respiratory syndrome
Severe acute respiratory syndrome coronavirus 2
Silver
Spike Glycoprotein, Coronavirus
Spike protein
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Summary:Dipstick assays using silver nanoparticles (AgNPs) stabilized by a thin calix[4]­arene-based coating were developed and used for the detection of Anti-SARS-CoV-2 IgG in clinical samples. The calixarene-based coating enabled the covalent bioconjugation of the SARS-CoV-2 Spike Protein via the classical EDC/sulfo-NHS procedure. It further conferred remarkable stability to the resulting bioconjugated AgNPs, as no degradation was observed over several months. In comparison with lateral-flow immunoassays (LFIAs) based on classical gold nanoparticles, our AgNP-based system constitutes a clear step forward, as the limit of detection for Anti-SARS-CoV-2 IgG was reduced by 1 order of magnitude and similar signals were observed with 10 times fewer particles. In real clinical samples, the AgNP-based dipstick assays showed impressive results: 100% specificity was observed for negative samples, while a sensitivity of 73% was determined for positive samples. These values match the typical sensitivities obtained for reported LFIAs based on gold nanoparticles. These results (i) represent one of the first examples of the use of AgNP-based dipstick assays in the case of real clinical samples, (ii) demonstrate that ultrastable calixarene-coated AgNPs could advantageously replace AuNPs in LFIAs, and thus (iii) open new perspectives in the field of rapid diagnostic tests.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.2c00870