SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet-plasma membrane tethering

Membrane contact sites (MCSs) are interorganellar connections that allow for the direct exchange of molecules, such as lipids or Ca2+ between organelles, but can also serve to tether organelles at specific locations within cells. Here, we identified and characterized three proteins of Arabidopsis th...

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Published in:The Plant cell 2022-05, Vol.34 (6), p.2424-2448
Main Authors: Krawczyk, Hannah Elisa, Sun, Siqi, Doner, Nathan M, Yan, Qiqi, Lim, Magdiel Sheng Satha, Scholz, Patricia, Niemeyer, Philipp William, Schmitt, Kerstin, Valerius, Oliver, Pleskot, Roman, Hillmer, Stefan, Braus, Gerhard H, Wiermer, Marcel, Mullen, Robert T, Ischebeck, Till
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
Cell Biology
Cell Membrane - metabolism
Lipid Droplets - metabolism
Plant Sciences
Seedlings - genetics
Seedlings - metabolism
Seeds - genetics
Seeds - metabolism
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Summary:Membrane contact sites (MCSs) are interorganellar connections that allow for the direct exchange of molecules, such as lipids or Ca2+ between organelles, but can also serve to tether organelles at specific locations within cells. Here, we identified and characterized three proteins of Arabidopsis thaliana that form a lipid droplet (LD)-plasma membrane (PM) tethering complex in plant cells, namely LD-localized SEED LD PROTEIN (SLDP) 1 and SLDP2 and PM-localized LD-PLASMA MEMBRANE ADAPTOR (LIPA). Using proteomics and different protein-protein interaction assays, we show that both SLDPs associate with LIPA. Disruption of either SLDP1 and SLDP2 expression, or that of LIPA, leads to an aberrant clustering of LDs in Arabidopsis seedlings. Ectopic co-expression of one of the SLDPs with LIPA is sufficient to reconstitute LD-PM tethering in Nicotiana tabacum pollen tubes, a cell type characterized by dynamically moving LDs in the cytosolic streaming. Furthermore, confocal laser scanning microscopy revealed both SLDP2.1 and LIPA to be enriched at LD-PM contact sites in seedlings. These and other results suggest that SLDP and LIPA interact to form a tethering complex that anchors a subset of LDs to the PM during post-germinative seedling growth in Arabidopsis.
ISSN:1040-4651
1532-298X
1532-298X
DOI:10.1093/plcell/koac095