The effect of transportation and lairage on faecal shedding and carcass contamination with Escherichia coli O157 and O26 in very young calves in New Zealand

• Published in: Epidemiology and infection 2018-07, Vol.146 (9), p.1089-1100
• Main Authors: Jaros, P., Cookson, A. L., Reynolds, A., Withers, H., Clemens, R., Brightwell, G., Mills, J., Marshall, J., Prattley, D. J., Campbell, D. M., Hathaway, S., French, N. P.
• Format: Article
• Language: English
• Subjects: Bacteria; Calves; Carcasses; Cattle; Contamination; Dairy farms; E coli; Escherichia coli; Farms; Food contamination & poisoning; Food processing industry; Gastrointestinal Infection; Hygiene; Meat processing; Mucosa; Original Paper; Pollution control; Shedding; Shiga toxin; Slaughter; Transportation
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S0950268818000973

The effect of transportation and lairage on the faecal shedding and post-slaughter contamination of carcasses with Escherichia coli O157 and O26 in young calves (4–7-day-old) was assessed in a cohort study at a regional calf-processing plant in the North Island of New Zealand, following 60 calves as cohorts from six dairy farms to slaughter. Multiple samples from each animal at pre-slaughter (recto-anal mucosal swab) and carcass at post-slaughter (sponge swab) were collected and screened using real-time PCR and culture isolation methods for the presence of E. coli O157 and O26 (Shiga toxin-producing E. coli (STEC) and non-STEC). Genotype analysis of E. coli O157 and O26 isolates provided little evidence of faecal–oral transmission of infection between calves during transportation and lairage. Increased cross-contamination of hides and carcasses with E. coli O157 and O26 between co-transported calves was confirmed at pre-hide removal and post-evisceration stages but not at pre-boning (at the end of dressing prior to chilling), indicating that good hygiene practices and application of an approved intervention effectively controlled carcass contamination. This study was the first of its kind to assess the impact of transportation and lairage on the faecal carriage and post-harvest contamination of carcasses with E. coli O157 and O26 in very young calves.