Development and Characterization of Chromosome Segment Substitution Lines Derived from Oryza rufipogon in the Background of the Oryza sativa indica Restorer Line R974

Dongxiang wild rice (DXWR) ( Griff.), which has the northernmost worldwide distribution of a wild rice species, is a valuable genetic resource with respect to improving stress tolerance in cultivated rice ( L.). In the three-line hybrid rice breeding system, restorer lines play important roles in en...

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Bibliographic Details
Published in:Genes 2022-04, Vol.13 (5), p.735
Main Authors: Ding, Gumu, Hu, Biaolin, Zhou, Yi, Yang, Wanling, Zhao, Minmin, Xie, Jiankun, Zhang, Fantao
Format: Article
Language:English
Subjects:
Abiotic stress
Chromosome 3
Chromosomes
Chromosomes, Plant - genetics
Cultivars
Genomes
Genomics
Geographical distribution
Germplasm
Oryza - genetics
Oryza rufipogon
Oryza sativa indica
Plant Breeding
Population
Quantitative Trait Loci
Rice
Salinity tolerance
Seeds
Single-nucleotide polymorphism
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Summary:Dongxiang wild rice (DXWR) ( Griff.), which has the northernmost worldwide distribution of a wild rice species, is a valuable genetic resource with respect to improving stress tolerance in cultivated rice ( L.). In the three-line hybrid rice breeding system, restorer lines play important roles in enhancing the tolerance of hybrid rice. However, restorer lines have yet to be used as a genomic background for development of substitution lines carrying DXWR chromosome segments. We developed a set of 84 chromosome segment substitution lines (CSSLs) from a donor parent DXWR × recurrent parent restorer line R974 ( ) cross. On average, each CSSL carried 6.27 introgressed homozygous segments, with 93.37% total genome coverage. Using these CSSLs, we identified a single QTL, associated with salt stress tolerance on chromosome 3. Furthermore, five CSSLs showing strong salt stress tolerance were subjected to whole-genome single-nucleotide polymorphism chip analyses, during which we detected a common substitution segment containing in all five CSSLs, thereby implying the validity and efficacy of . These novel CSSLs could make a significant contribution to detecting valuable DXWR QTLs, and provide important germplasm resources for breeding novel restorer lines for use in hybrid rice breeding systems.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes13050735