Application of Confocal Raman Microscopy for the Characterization of Topical Semisolid Formulations and their Penetration into Human Skin Ex Vivo

• Published in: Pharmaceutical research 2022-05, Vol.39 (5), p.935-948
• Main Authors: Jung, Nathalie, Namjoshi, Sarika, Mohammed, Yousuf, Grice, Jeffrey E., Benson, Heather A. E., Raney, Sam G., Roberts, Michael S., Windbergs, Maike
• Format: Article
• Language: English
• Subjects: Acyclovir; Antiviral drugs; Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Cryopreservation; Customer relationship management software; Drug Compounding - methods; Drug delivery; Excipients - analysis; Humans; Medical Law; Microscope and microscopy; Microscopy; Microscopy, Confocal - methods; Pharmaceutical industry; Pharmacology/Toxicology; Pharmacy; Research Paper; Skin; Skin - metabolism; Skin Absorption; Spectrum Analysis, Raman - methods; Transdermal medication
• ISSN: 0724-8741; 1573-904X; 1573-904X
• DOI: 10.1007/s11095-022-03245-7

Purpose The quality testing and approval procedure for most pharmaceutical products is a streamlined process with standardized procedures for the determination of critical quality attributes. However, the evaluation of semisolid dosage forms for topical drug delivery remains a challenging task. The work presented here highlights confocal Raman microscopy (CRM) as a valuable tool for the characterization of such products. Methods CRM, a laser-based method, combining chemically-selective analysis and high resolution imaging, is used for the evaluation of different commercially available topical acyclovir creams. Results We show that CRM enables the spatially resolved analysis of microstructural features of semisolid products and provides insights into drug distribution and polymorphic state as well as the composition and arrangement of excipients. Further, we explore how CRM can be used to monitor phase separation and to study skin penetration and the interaction with fresh and cryopreserved excised human skin tissue. Conclusion This study presents a comprehensive overview and illustration of how CRM can facilitate several types of key analyses of semisolid topical formulations and of their interaction with their biological target site, illustrating that CRM is a useful tool for research, development as well as for quality testing in the pharmaceutical industry. Graphical abstract