Typing of Salmonella enterica serovar Infantis isolates from 51 outbreaks in Germany between 1974 and 2009 by a novel phage-typing scheme

We developed a new phage-typing method and evaluated its application in combination with XbaI macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) as a useful tool for the long-term epidemiology of Salmonella enterica serovar Infantis. In this study, we investigated 1008 S. Infantis...

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Bibliographic Details
Published in:Epidemiology and infection 2014-01, Vol.142 (1), p.75-83
Main Authors: MILLER, T., BRAUN, P. G., FEHLHABER, K., PRAGER, R., PFEIFER, Y., RABSCH, W.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Antimicrobials
Bacteriology
Bacteriophage typing
Bacteriophage Typing - methods
Bacteriophages
Biological and medical sciences
Broiler chickens
Cattle
Chickens
Disease Outbreaks
Dogs
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Epidemics
Epidemiology
European Union
Food
Food Microbiology
Fundamental and applied biological sciences. Psychology
Gastroenteritis
Gastroenteritis/Water
Germany - epidemiology
Hogs
Humans
Infections
Meat - microbiology
Microbial Sensitivity Tests
Microbiology
Miscellaneous
Original Papers
Salmonella
Salmonella enterica
Salmonella enterica - classification
Salmonella enterica - drug effects
Salmonella enterica - isolation & purification
Salmonella enterica - virology
Salmonella Infections - epidemiology
Salmonella Infections - microbiology
Surveillance
Virology
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Summary:We developed a new phage-typing method and evaluated its application in combination with XbaI macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) as a useful tool for the long-term epidemiology of Salmonella enterica serovar Infantis. In this study, we investigated 1008 S. Infantis isolates recovered from humans, various animal species and food products from 1973 to 2009. The typing scheme is based on 17 typing phages, defining 61 different patterns within the strain collection. The experiments showed that phage typing is a reliable method for differentiation of outbreaks and sporadic clinical cases as well as for elucidation of chains of transmission. The combined analysis of phage typing and PFGE revealed the existence of epidemic clones with a high stability over time like PT29/XB27 which was identified in nosocomial salmonellosis, community outbreaks as well as in broiler chickens from 2002 to 2009.
ISSN:0950-2688
1469-4409
1469-4409
DOI:10.1017/S095026881300037X