Ungulate bocaparvovirus 4 and rodent bocavirus are different genotypes of the same species of virus

• Published in: Virologica Sinica 2022-04, Vol.37 (2), p.215-222
• Main Authors: He, Wenqiao, Gao, Yuhan, Wen, Yuqi, Ke, Xuemei, Ou, Zejin, Fu, Jiaqi, Cheng, Mingji, Mo, Yun, Chen, Qing
• Format: Article
• Language: English
• Subjects: Genome; Rats; Rodent bocavirus (RoBoV); Ungulate bocaparvovirus 4
• ISSN: 1995-820X; 1674-0769; 1995-820X
• DOI: 10.1016/j.virs.2022.02.002

Bocaviruses are associated with many human infectious diseases, such as respiratory tract infections, gastroenteritis, and hepatitis. Rats are known to be reservoirs of bocaviruses, including rodent bocavirus and rat bocavirus. Recently, ungulate bocaparvovirus 4, a known porcine bocavirus, has also been found in rats. Thus, investigating bocaviruses in rats is important for determining the origin of the viruses and preventing and controlling their transmission. To the best of our knowledge, no study to date has investigated bocaviruses in the livers of rats. In this report, a total of 624 rats were trapped in southern China between 2014 and 2017. Liver and serum samples from rats were tested for the prevalence of bocaviruses using PCR. Sequences related to ungulate bocaparvovirus 4 and rodent bocavirus were detected in both liver and serum samples. Interestingly, the prevalence of ungulate bocaparvovirus 4 (reference strain: KJ622366.1) was higher than that of rodent bocavirus (reference strain: KY927868.1) in both liver (2.24% and 0.64%, respectively) and serum samples (2.19% and 0.44%, respectively). The NS1 regions of ungulate bocaparvovirus 4 and rodent bocavirus related sequences displayed over 84% and 88% identity at the nucleic acid and amino acid levels, respectively. Furthermore, these sequences had similar genomic structure, genomic features, and codon usage bias, and shared a common ancestor. These viruses also displayed greater adaptability to rats than pigs. Our results suggested that ungulate bocaparvovirus 4 and rodent bocavirus may originate from rats and may be different genotypes of the same bocavirus species. •This is the first study to investigate bocaviruses in the liver of rats.•The prevalence of bocaviruses were investigated in rats from southern China between 2014 and 2017.•The prevalence of ungulate bocaparvovirus 4 was higher than that of RoBoV in both liver and serum samples.•Ungulate bocaparvovirus 4 and RoBoV share high genomic structure and features, and codon usage bias.