Characterization of unexpected growth of Escherichia coli O157:H7 by modeling

Modeling of batch kinetics in minimal synthetic medium was used to characterize Escherichia coli O157:H7 growth, which appeared to be different from the exponential growth expected in minimal synthetic medium and observed for E. coli K-12. The turbidimetric kinetics of 14 of the 15 O157:H7 strains t...

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Bibliographic Details
Published in:Applied and environmental microbiology 1999-12, Vol.65 (12), p.5322-5327
Main Authors: CORNU, M, DELIGNETTE-MULLER, M. L, FLANDROIS, J.-P
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacteriology
Biological and medical sciences
Cattle
Culture Media
Escherichia coli
Escherichia coli - classification
Escherichia coli - growth & development
Escherichia coli O157 - growth & development
Escherichia coli O157 - isolation & purification
Feces - microbiology
Food Microbiology
Fundamental and applied biological sciences. Psychology
Humans
Kinetics
Life Sciences
Mathematical models
Meat - microbiology
Microbiology
Milk - microbiology
Models, Biological
Other
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Physical growth
Species Specificity
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Summary:Modeling of batch kinetics in minimal synthetic medium was used to characterize Escherichia coli O157:H7 growth, which appeared to be different from the exponential growth expected in minimal synthetic medium and observed for E. coli K-12. The turbidimetric kinetics of 14 of the 15 O157:H7 strains tested (93%) were nonexponential, whereas 25 of the 36 other E. coli strains tested (70%) exhibited exponential kinetics. Moreover, the anomaly was almost corrected when the minimal medium was supplemented with methionine. These observations were confirmed with two reference strains by using plate count monitoring. In mixed cultures, E. coli K-12 had a positive effect on E. coli O157:H7 and corrected its growth anomaly. This demonstrated that commensalism occurred, as the growth curve for E. coli K-12 was not affected. The interaction could be explained by an exchange of methionine, as the effect of E. coli K-12 on E. coli O157:H7 appeared to be similar to the effect of methionine.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.65.12.5322-5327.1999