Diffusion-Weighted Imaging to Assess HPV-Positive versus HPV-Negative Oropharyngeal Squamous Cell Carcinoma: The Importance of b-Values

• Published in: American journal of neuroradiology : AJNR 2022-06, Vol.43 (6), p.905-912
• Main Authors: Lenoir, V, Delattre, B M A, M'RaD, Y, De Vito, C, de Perrot, T, Becker, M
• Format: Article
• Language: English
• Subjects: Carcinoma, Squamous Cell - diagnostic imaging; Carcinoma, Squamous Cell - pathology; Diffusion Magnetic Resonance Imaging - methods; Functional; Head & Neck; Head and Neck Neoplasms; Humans; Papillomavirus Infections - complications; Papillomavirus Infections - diagnostic imaging; Papillomavirus Infections - pathology; Squamous Cell Carcinoma of Head and Neck
• ISSN: 0195-6108; 1936-959X
• DOI: 10.3174/ajnr.A7521

Controversy exists as to whether ADC histograms are capable to distinguish human papillomavirus-positive (HPV+) from human papillomavirus-negative (HPV-) oropharyngeal squamous cell carcinoma. We investigated how the choice of b-values influences the capability of ADC histograms to distinguish between the two tumor types. Thirty-four consecutive patients with histologically proved primary oropharyngeal squamous cell carcinoma (11 HPV+ and 23 HPV-) underwent 3T MR imaging with a single-shot EPI DWI sequence with 6 b-values (0, 50, 100, 500, 750, 1000 s/mm ). Monoexponentially calculated perfusion-sensitive (including =0 s/mm ) and perfusion-insensitive/true diffusion ADC maps (with b ≥ 100 s/mm as the lowest b-value) were generated using Matlab. The choice of b-values included 2 b-values (ADC , ADC , ADC , ADC ) and 3-6 b-values (ADC , ADC , ADC , ADC , ADC ). Readers blinded to the HPV- status contoured all tumors. ROIs were then copied onto ADC maps, and their histograms were compared. ADC histogram metrics in HPV+ and HPV- oropharyngeal squamous cell carcinoma changed significantly depending on the b-values. The mean ADC was lower, and skewness was higher in HPV+ than in HPV- oropharyngeal squamous cell carcinoma only for ADC ADC , and ADC ( < .05), allowing distinction between the 2 tumor types. Kurtosis was significantly higher in HPV+ versus HPV- oropharyngeal squamous cell carcinoma for all b-value combinations except 2 perfusion-insensitive maps (ADC and ADC ). Among all b-value combinations, kurtosis on ADC had the highest diagnostic performance to distinguish HPV+ from HPV- oropharyngeal squamous cell carcinoma (area under the curve = 0.893; sensitivity = 100%, specificity = 82.6%). Acquiring multiple b-values for ADC calculation did not improve the distinction between HPV+ and HPV- oropharyngeal squamous cell carcinoma. The choice of b-values significantly affects ADC histogram metrics in oropharyngeal squamous cell carcinoma. Distinguishing HPV+ from HPV- oropharyngeal squamous cell carcinoma is best possible on the ADC map.