Influence of different functional elements of plasmid pGT232 on maintenance of recombinant plasmids in Lactobacillus reuteri populations in vitro and in vivo

Plasmid pGT232 (5.1 kb), an indigenous plasmid of Lactobacillus reuteri 100-23, was determined, on the basis of nucleotide and deduced protein sequence data, to belong to the pC194-pUB110 family of plasmids that replicate via the rolling-circle mechanism. The minimal replicon of pGT232 was located o...

Full description

Saved in:
Bibliographic Details
Published in:Applied and environmental microbiology 1999-12, Vol.65 (12), p.5378-5385
Main Authors: HENG, N. C. K, BATEUP, J. M, LOACH, D. M, XIYANG WU, JENKINSON, H. F, MORRISON, M, TANNOCK, G. W
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria
Base Sequence
Biological and medical sciences
Biotechnology
Cellular biology
Cloning, Molecular
DNA Replication
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetics and Molecular Biology
Lactobacillus - genetics
Lactobacillus reuteri
Methods. Procedures. Technologies
Microbiology
Molecular Sequence Data
plasmid pC194
plasmid pGT232
plasmid pUB110
Plasmids
Recombination, Genetic
repA gene
Replicon - genetics
Restriction Mapping
Sequence Deletion
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plasmid pGT232 (5.1 kb), an indigenous plasmid of Lactobacillus reuteri 100-23, was determined, on the basis of nucleotide and deduced protein sequence data, to belong to the pC194-pUB110 family of plasmids that replicate via the rolling-circle mechanism. The minimal replicon of pGT232 was located on a 1.7-kb sequence consisting of a double-strand origin of replication and a gene encoding the replication initiation protein, repA. An erythromycin-selectable recombinant plasmid containing this minimal replicon was stably maintained (>97% erythromycin-resistant cells) without antibiotic selection in an L. reuteri population under laboratory growth conditions but was poorly maintained (90% resistant cells) of pGT232-derived plasmids in the lactobacillus population in vivo required an additional 1.0-kb sequence which contained a putative single-strand replication origin (SSO). The SSO of pGT232 is believed to be novel and functions in an orientation-specific manner.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.65.12.5378-5385.1999