Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension

During the process of tumor growth, cancer cells will be subjected to intermittent hypoxia. This results from the delay in the development of the vascular network in relation to the proliferation of cancer cells. The hypoxic nature of a tumor has been demonstrated as a negative factor for patient su...

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Bibliographic Details
Published in:Oncology letters 2022-07, Vol.24 (1), p.1, Article 220
Main Authors: Chabaud, Stephane, Pellerin, Eve, Caneparo, Christophe, Ringuette-Goulet, Cassandra, Pouliot, Frederic, Bolduc, Stephane
Format: Article
Language:English
Subjects:
Antibodies
Binding sites
Biology
Bladder cancer
Cancer
Cancer therapies
Cell culture
Cell death
Experiments
Extracellular matrix
Hypoxia
Medical research
Metabolism
Metastasis
Oncology, Experimental
Physiological aspects
Proteins
White people
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Summary:During the process of tumor growth, cancer cells will be subjected to intermittent hypoxia. This results from the delay in the development of the vascular network in relation to the proliferation of cancer cells. The hypoxic nature of a tumor has been demonstrated as a negative factor for patient survival. To evaluate the impact of hypoxia on the survival and migration properties of low and high-grade bladder cancer cell lines, two low-grade (MGHU-3 and SW-780) and two high-grade (SW-1710 and T24) bladder cancer cell lines were cultured in normoxic (20% [O.sub.2]) or hypoxic atmospheric conditions (2% [O.sub.2]). The response of bladder cancer cell lines to hypoxic atmospheric cell culture conditions was examined under several parameters, including epithelial-mesenchymal transition, doubling time and metabolic activities, throm-bospondin-1 expression, whole Matrix Met allo-Proteinase activity, migration and resistance to oxidative stress. The low-grade cell line response to hypoxia was heterogeneous even if it tended to adopt a more aggressive profile. Hypoxia enhanced migration and pro-survival properties of MGHU-3 cells, whereas these features were reduced for the SW-780 cell line cultured under low oxygen tension. The responses of tested high-grade cell lines were more homogeneous and tended to adopt a less aggressive profile. Hypoxia drastically changed some of the bladder cancer cell line properties, for example matrix met alloproteinases expression for all cancer cells but also switch in glycolytic metabolism of low grade cancer cells. Overall, studying bladder cancer cells in hypoxic environments are relevant for the translation from in vitro findings to in vivo context.
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2022.13341