CkREV Enhances the Drought Resistance of Caragana korshinskii through Regulating the Expression of Auxin Synthetase Gene CkYUC5

As a common abiotic stress, drought severely impairs the growth, development, and even survival of plants. Here we report a transcription factor, REVOLUTA(CkREV), which can bidirectionally regulate the expression of the critical enzyme gene in auxin synthesis according to external environment change...

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Published in:International journal of molecular sciences 2022-05, Vol.23 (11), p.5902
Main Authors: Li, Jia-Yang, Ren, Jie-Jie, Zhang, Tian-Xin, Cui, Jin-Hao, Gong, Chun-Mei
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - metabolism
Auxins
Biosynthesis
Caragana - genetics
Caragana korshinskii
Drought
Drought resistance
Droughts
Enzymes
Evolution & development
Gene expression
Gene Expression Regulation, Plant
Genes
Growth rate
Hormones
Indoleacetic Acids - metabolism
Ligases - metabolism
Phylogenetics
Plant growth
Plants, Genetically Modified - metabolism
Stress, Physiological - genetics
Transcription factors
Verification
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Summary:As a common abiotic stress, drought severely impairs the growth, development, and even survival of plants. Here we report a transcription factor, REVOLUTA(CkREV), which can bidirectionally regulate the expression of the critical enzyme gene in auxin synthesis according to external environment changes, so as to control the biosynthesis of auxin and further enhance the drought resistance of plants. Quantitative analysis reveals that the expression level of both and is down-regulated after and are exposed to drought. Functional verification of CkREV reveals that CkREV up-regulates the expression of in transgenic under common conditions, while down-regulating it under drought conditions. Meanwhile, the expression of is also down-regulated in leaves instantaneously overexpressing CkREV. We apply a dual-luciferase reporter system to discover that CkREV can bind to the promoter of to regulate its expression, which is further proved by EMSA and Y1H esxperiments. Functional verification of CkREV in and transgenic shows that CkREV can regulate the expression of and in a contrary way, maintaining the equilibrium of plants between growth and drought resisting. CkREV can positively regulate the expression of to promote auxin synthesis in favor of growth under normal development. However, CkREV can also respond to external signals and negatively regulate the expression of , which inhibits auxin synthesis in order to reduce growth rate, lower water demands, and eventually improve the drought resistance of plants.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23115902