Impact of Polyrhodanine Manganese Ferrite Binary Nanohybrids (PRHD@MnFe 2 O 4 ) on Osteoblasts and Osteoclasts Activities-A Key Factor in Osteoporosis Treatment

Osteoporosis is characterized by the reduction of bone mineral density and the weakness of the bone strength leading to fractures. Searching for new compounds that stimulate bone activity and their ability to reconstruct seems to be a promising tool in osteoporosis treatment. Here, we performed anal...

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Bibliographic Details
Published in:Materials 2022-06, Vol.15 (11), p.3990
Main Authors: Marycz, Krzysztof, Kowalczuk, Anna, Turlej, Eliza, Zachanowicz, Emilia, Tomaszewska, Anna, Kulpa-Greszta, Magdalena, Kępska, Martyna, Steczkiewicz, Zofia, Pązik, Robert
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Apoptosis
Biomedical materials
Bone density
Differentiation (biology)
Extracellular matrix
Fourier transforms
Fractures
Gene expression
Genes
Homeostasis
Impact analysis
Ligands
Manganese
Mineralization
Nanoparticles
Osteoblasts
Osteoporosis
Polymerization
Proteins
Spectrum analysis
Tumor necrosis factor-TNF
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Summary:Osteoporosis is characterized by the reduction of bone mineral density and the weakness of the bone strength leading to fractures. Searching for new compounds that stimulate bone activity and their ability to reconstruct seems to be a promising tool in osteoporosis treatment. Here, we performed analyses comparing the impact of polyrhodanine (PRHD) and its derivatives on the viability (anti-proliferative tests), morphology and mitochondrial network (confocal microscopy) towards pre-osteoblasts (MC3T3-E1 cell line) and osteoclasts (4B12 cell line). Moreover, we assessed the expression of genes associated with the apoptosis, inflammation and osteogenic differentiation by qPCR technique. Our results clearly demonstrated that PRHD and its modification at ratio 10/90 significantly improves the pre-osteoblast's proliferative abilities, while reducing osteoclast function. The observed effects were strongly correlated with the cytoskeleton and mitochondrial network development and arrangement. Additionally, the expression profile of genes revealed enhanced apoptosis of osteoclasts in the case of PRHD and its modification at ratio 10/90. Moreover, in this case we also observed strong anti-inflammatory properties demonstrated by decreased expression of and in pre-osteoblasts and osteoclasts. On the other hand, enhanced expression of the markers associated with bone remodeling, namely, osteopontin (OPN), osteocalcin (OCL) and alkaline phosphatase (ALP), seem to confirm the role of PRHD@MnFe O in the promotion of differentiation of pre-osteoblasts through the axis. Based on these observations, PRHD@MnFe O could be a potential agent in osteoporosis treatment in future, however, further studies are still required.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma15113990