Draft Genomes of Nitrogen-fixing Frankia Strains Ag45/Mut15 and AgPM24 Isolated from Root Nodules of Alnus Glutinosa

The genomes of two nitrogen-fixing strains, Ag45/Mut15 and AgPM24, isolated from root nodules of are described as representatives of a novel candidate species Phylogenomic and ANI analyses confirmed that both strains are related to cluster 1 frankiae, and that both strains belong to a novel species....

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Bibliographic Details
Published in:Journal of genomics 2022, Vol.10, p.49-56
Main Authors: Normand, Philippe, Pujic, Petar, Abrouk, Danis, Vemulapally, Spandana, Guerra, Trina, Carlos-Shanley, Camila, Hahn, Dittmar
Format: Article
Language:English
Subjects:
Bacteriology
Biochemistry, Molecular Biology
Genomics
Life Sciences
Microbiology and Parasitology
Research Paper
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Summary:The genomes of two nitrogen-fixing strains, Ag45/Mut15 and AgPM24, isolated from root nodules of are described as representatives of a novel candidate species Phylogenomic and ANI analyses confirmed that both strains are related to cluster 1 frankiae, and that both strains belong to a novel species. At 6.4 - 6.7 Mb, their genomes were smaller than those of other cultivated -infective cluster 1 strains but larger than that of the non-cultivated -infective cluster 1 Sp+ strain AgTrS that was their closest neighbor as assessed by ANI. Comparative genomic analyses identified genes essential for nitrogen-fixation, gene composition as regards COGs, secondary metabolites clusters and transcriptional regulators typical of those from -infective cluster 1 cultivated strains in both genomes. There were 459 genes present in other cultivated -infective strains lost in the two genomes, spread over the whole of the genome, which indicates genome erosion is taking place in these two strains.
ISSN:1839-9940
1839-9940
DOI:10.7150/jgen.74788