Principles and Problems of Exosome Isolation from Biological Fluids

Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic and therapeutic potential, but the lack of standardized methods for their efficient isolation and analysis limits the introduction of exosomal technologies into clinical practice. This review discusses the problem...

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Bibliographic Details
Published in:Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2022, Vol.16 (2), p.115-126
Main Authors: Yakubovich, E. I., Polischouk, A. G., Evtushenko, V. I.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Exosomes
Life Sciences
Membrane vesicles
Principles
Reviews
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Summary:Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic and therapeutic potential, but the lack of standardized methods for their efficient isolation and analysis limits the introduction of exosomal technologies into clinical practice. This review discusses the problems associated with the isolation of exosomes from biological fluids, as well as the principles of traditional and alternative methods of isolation. The aim of the presented review is to illustrate the variety of approaches based on the physical and biochemical properties of exosomes that can be used for exosome isolation. The advantages and disadvantages of different methods are discussed.
ISSN:1990-7478
1990-7494
DOI:10.1134/S1990747822030096