The phagocytic cyst cells in Drosophila testis eliminate germ cell progenitors via phagoptosis

Phagoptosis is a frequently occurring nonautonomous cell death pathway in which phagocytes eliminate viable cells. While it is thought that phosphatidylserine (PS) “eat-me” signals on target cells initiate the process, the precise sequence of events is largely unknown. Here, we show that in Drosophi...

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Published in:Science advances 2022-06, Vol.8 (24), p.eabm4937-eabm4937
Main Authors: Zohar-Fux, Maayan, Ben-Hamo-Arad, Aya, Arad, Tal, Volin, Marina, Shklyar, Boris, Hakim-Mishnaevski, Ketty, Porat-Kuperstein, Lilach, Kurant, Estee, Toledano, Hila
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container_issue 24
container_start_page eabm4937
container_title Science advances
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creator Zohar-Fux, Maayan
Ben-Hamo-Arad, Aya
Arad, Tal
Volin, Marina
Shklyar, Boris
Hakim-Mishnaevski, Ketty
Porat-Kuperstein, Lilach
Kurant, Estee
Toledano, Hila
description Phagoptosis is a frequently occurring nonautonomous cell death pathway in which phagocytes eliminate viable cells. While it is thought that phosphatidylserine (PS) “eat-me” signals on target cells initiate the process, the precise sequence of events is largely unknown. Here, we show that in Drosophila testes, progenitor germ cells are spontaneously removed by neighboring cyst cells through phagoptosis. Using live imaging with multiple markers, we demonstrate that cyst cell–derived early/late endosomes and lysosomes fused around live progenitors to acidify them, before DNA fragmentation and substantial PS exposure on the germ cell surface. Furthermore, the phagocytic receptor Draper is expressed on cyst cell membranes and is necessary for phagoptosis. Significantly, germ cell death is blocked by knockdown of either the endosomal component Rab5 or the lysosomal associated protein Lamp1, within the cyst cells. These data ascribe an active role for phagocytic cyst cells in removal of live germ cell progenitors. During spermatogenesis, phagocytes execute excessive live germ cell progenitors without any evidential provocation.
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Cell Biology
Developmental Biology
SciAdv r-articles
title The phagocytic cyst cells in Drosophila testis eliminate germ cell progenitors via phagoptosis
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