Dissemination of methicillin-resistant Staphylococcus aureus SCCmec type IV and SCCmec type V epidemic clones in a tertiary hospital: challenge to infection control

Two-hundred MRSA strains from inpatients with healthcare-associated (HA) and 100 MRSA strains from outpatients with community-associated (CA) skin and soft tissue infections (SSTIs) were tested for antimicrobial susceptibility, staphylococcal cassette chromosome mec (SCCmec) typing, Panton–Valentine...

Full description

Saved in:
Bibliographic Details
Published in:Epidemiology and infection 2015-01, Vol.143 (2), p.343-353
Main Authors: DHAWAN, B., RAO, C., UDO, E. E., GADEPALLI, R., VISHNUBHATLA, S., KAPIL, A.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacterial Proteins - genetics
Bacterial Toxins - genetics
Child
Cloning
Cross Infection - epidemiology
Cross Infection - microbiology
Cross Infection - prevention & control
Drug resistance
Enterotoxins - genetics
Epidemiology
Exotoxins - genetics
Female
Genes
Humans
Infection Control
Leukocidins - genetics
Male
Methicillin Resistance - genetics
Methicillin-Resistant Staphylococcus aureus - classification
Methicillin-Resistant Staphylococcus aureus - drug effects
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - isolation & purification
Microbial Sensitivity Tests
Middle Aged
Multilocus Sequence Typing
Nosocomial infections
Original Papers
Patients
Sepsis
Staphylococcal Infections - epidemiology
Staphylococcal Infections - microbiology
Staphylococcal Infections - prevention & control
Staphylococcus aureus
Staphylococcus infections
Tertiary Care Centers - statistics & numerical data
Young Adult
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two-hundred MRSA strains from inpatients with healthcare-associated (HA) and 100 MRSA strains from outpatients with community-associated (CA) skin and soft tissue infections (SSTIs) were tested for antimicrobial susceptibility, staphylococcal cassette chromosome mec (SCCmec) typing, Panton–Valentine leucocidin (PVL) toxin, seh and arcA genes. Based on SCCmec typing, HA-MRSA isolates were further divided into HA-SCCmec I/II/III MRSA and HA-SCCmec IV/V MRSA, and CA-MRSA isolates into CA-SCCmec I/II/III MRSA and CA-SCCmec IV/V MRSA. SCCmec types were further characterized by pulsed-field gel electrophoresis, spa typing and multi-locus sequence typing. Seventy-five (37·5%) HA-MRSA isolates and 83/100 CA-MRSA isolates were SCCmec IV/V genotype. HA-SCCmec IV/V MRSA was associated with malignancy (P = 0·03) and bone fractures (P = 0·02) compared to CA-SCCmec IV/V MRSA. HA-SCCmec IV/V MRSA was associated with PVL gene carriage compared to HA-SCCmec I/II/III MRSA (P 
ISSN:0950-2688
1469-4409
DOI:10.1017/S095026881400065X