Comprehensive Integrated Single-Cell Whole Transcriptome Analysis Revealed the p-EMT Tumor Cells—CAFs Communication in Oral Squamous Cell Carcinoma

Cancer-associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p-EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single-cell RNA sequencing datasets...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-06, Vol.23 (12), p.6470
Main Authors: Huynh, Nam Cong-Nhat, Huang, Tze-Ta, Nguyen, Chi Thi-Kim, Lin, Fang-Kuei
Format: Article
Language:English
Subjects:
Antigens
Cancer
Cell cycle
Cell division
Communication
Cytokines
Fibroblasts
Gene expression
Investigations
Lymphatic system
Lymphocytes
Medical screening
Mesenchyme
Metastases
Metastasis
Oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Proteins
Squamous cell carcinoma
Transcriptomes
Tumor cells
Tumorigenesis
Tumors
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Summary:Cancer-associated fibroblasts (CAFs) and partial epithelial–mesenchymal transition (p-EMT) tumor cells are closed together and contribute to the tumor progression of oral squamous cell carcinoma (OSCC). In the present study, we deeply analyzed and integrated OSCC single-cell RNA sequencing datasets to define OSCC CAFs and p-EMT subpopulations. We highlighted the cell–cell interaction network of CAFs and p-EMT tumor cells and suggested biomarkers for the diagnosis and prognosis of OSCC during the metastasis condition. The analysis discovered four subtypes of CAFs: one p-EMT tumor cell population, and cycling tumor cells as well as TNFSF12-TNFRSF25/TNFRSF12A interactions between CAFs and p-EMT tumor cells during tumor metastasis. This suggests the prediction of therapeutically targetable checkpoint receptor–ligand interactions between CAFs and p-EMT tumor cells in OSCC regarding the metastasis status.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23126470