Antisense silencing of the creA gene in Aspergillus nidulans

Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approxi...

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Bibliographic Details
Published in:Applied and environmental microbiology 2000-10, Vol.66 (10), p.4579-4581
Main Authors: BAUTISTA, L. Fernando, ALEKSENKO, Alexei, HENTZER, Morten, SANTERRE-HENRIKSEN, Anne, NIELSEN, Jens
Format: Article
Language:English
Subjects:
alpha-Amylases - genetics
alpha-Amylases - metabolism
Aspergillus nidulans
Aspergillus nidulans - genetics
Aspergillus nidulans - growth & development
Aspergillus nidulans - metabolism
Biological and medical sciences
Biotechnology
creA gene
Enzymes
Fermentation
Fundamental and applied biological sciences. Psychology
Fungal Proteins
Fungi
Gene Expression Regulation, Bacterial - drug effects
Gene Silencing
Genes
Genetic engineering
Genetic technics
Glucose
Glucose - metabolism
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Methods. Procedures. Technologies
Microbiology
Modification of gene expression level
Mycology
Repressor Proteins - genetics
RNA, Antisense - pharmacology
Transcription, Genetic - drug effects
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Summary:Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.66.10.4579-4581.2000