LncRNA BC083743 Silencing Exacerbated Osteoporosis by Regulating the miR-103-3p/SATB2 Axis to Inhibit Osteogenic Differentiation

• Published in: Computational intelligence and neuroscience 2022-06, Vol.2022, p.7066759-9
• Main Authors: Lu, Feng, Tang, Ling
• Format: Article
• Language: English
• Subjects: Alizarin; Alkaline phosphatase; Arthritis; Binding sites; Biomedical materials; Bone marrow; Bone morphogenetic protein 2; Cbfa-1 protein; Cell culture; Cell cycle; Cells, Cultured; Differentiation (biology); Enzymes; Fractures; Gene expression; Humans; Immunoblotting; Matrix Attachment Region Binding Proteins; Mesenchyme; MicroRNAs - genetics; MicroRNAs - metabolism; Mineralization; Non-coding RNA; Osteogenesis; Osteogenesis - genetics; Osteoporosis; Osteoporosis - genetics; Osteoprotegerin; Patients; Phosphatases; Proteins; Reporter gene; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Long Noncoding - pharmacology; siRNA; Stem cells; Transcription Factors - pharmacology; Transfection
• ISSN: 1687-5265; 1687-5273
• DOI: 10.1155/2022/7066759

Objective. The target of the present paper was to reveal the influence of LncRNA BC083743 on osteogenesis in human bone marrow mesenchymal stem cells (hBMSCs). Methods. Serum specimens from osteoporotic patients and normal subjects were collected to isolate hBMSCs from femoral head tissue. The levels of BC083743 and miR-103-3p in serum and hBMSCs were measured by QRT-PCR. Alkaline phosphatase (ALP) activity test and alizarin red dyeing were used to identify ALP activity and mineralization forming ability of hBMSCs after transfection with si-BC083743 (siRNA-targeting BC083743). In addition, QRT-PCR and immunoblotting were conducted to identify the expressing levels of Runt-related transcription factor 2(Runx2), osteoprotegerin (OPG), and bone morphogenetic protein 2 (BMP2) in hBMSCs. Dual-luciferase reporter gene and RNA pull-down assays were employed to substantiate the binding of BC083743 to miR-103-3p and miR-103-3p to SATB2. Results. BC083743 expression was significantly downregulated in sera from patients with osteoporosis, and osteogenic differentiation-related genes and BC083743 expression were obviously upregulated as the time to osteogenic differentiation increased. BC083743 knockdown hindered the osteogenic differentiation of hBMSCs. BC083743 was aimed at miR-103-3p and miR-103-3p inhibitors partially reversed the inhibitory effect of BC083743 downregulation on hBMSCs osteogenesis. BC083743 silencing downregulated SATB2 through uptake of miR-103-3p, thereby inhibiting hBMSCs osteogenesis to exacerbate osteoporosis. Conclusion. BC083743/miR-103-3p/SATB2 axis inhibited osteogenic differentiation and exacerbated osteoporosis, which may offer brand-new molecular aims for the treatment of clinical osteoporosis.