Metabolic signatures of pregnancy-induced cardiac growth

• Published in: American journal of physiology. Heart and circulatory physiology 2022-07, Vol.323 (1), p.H146-H164
• Main Authors: Fulghum, Kyle L, Smith, Juliette B, Chariker, Julia, Garrett, Lauren F, Brittian, Kenneth R, Lorkiewicz, Pawel K, McNally, Lindsey A, Uchida, Shizuka, Jones, Steven P, Hill, Bradford G, Collins, Helen E
• Format: Article
• Language: English
• Subjects: Angiogenesis; Animals; Cardiac output; Cardiomegaly - metabolism; Cell proliferation; Dehydrogenase; Dehydrogenases; Fatty acids; Female; Heart; Hypertrophy; Ketones; Kinases; Metabolism; Metabolites; Metabolomics; Mice; Mitochondria; Mitochondria, Heart - metabolism; Muscle contraction; Myocardium - metabolism; Myocytes; Nitric oxide; Oxidation; Oxidation-Reduction; Phosphofructokinase; Phospholipids; Polyamines; Pregnancy; Proteomics; Pyruvic acid; Transcriptomics; Urea; Ventricle
• ISSN: 0363-6135; 1522-1539
• DOI: 10.1152/AJPHEART.00105.2022

The goal of this study was to develop an atlas of the metabolic, transcriptional, and proteomic changes that occur with pregnancy in the maternal heart. Timed pregnancy studies in FVB/NJ mice revealed a significant increase in heart size by of pregnancy (midpregnancy; MP), which was sustained throughout the rest of the term compared with nonpregnant control mice. Cardiac hypertrophy and myocyte cross-sectional area were highest 7 days after birth (postbirth; PB) and were associated with significant increases in end-diastolic and end-systolic left ventricular volumes and higher cardiac output. Metabolomics analyses revealed that by of pregnancy (late pregnancy; LP) metabolites associated with nitric oxide production as well as acylcholines, sphingomyelins, and fatty acid species were elevated, which coincided with a lower activation state of phosphofructokinase and higher levels of pyruvate dehydrogenase kinase 4 (Pdk4) and β-hydroxybutyrate dehydrogenase 1 (Bdh1). In the postpartum period, urea cycle metabolites, polyamines, and phospholipid levels were markedly elevated in the maternal heart. Cardiac transcriptomics in LP revealed significant increases in not only and but also genes that regulate glutamate and ketone body oxidation, which were preceded in MP by higher expression of transcripts controlling cell proliferation and angiogenesis. Proteomics analysis of the maternal heart in LP and PB revealed significant reductions in several contractile filament and mitochondrial subunit complex proteins. Collectively, these findings describe the coordinated molecular changes that occur in the maternal heart during and after pregnancy. Little is known of the underlying molecular and cellular mechanisms that contribute to pregnancy-induced cardiac growth. Several lines of evidence suggest that changes in cardiac metabolism may contribute. Here, we provide a comprehensive metabolic atlas of the metabolomic, proteomic, and transcriptomic changes occurring in the maternal heart. We show that pregnancy-induced cardiac growth is associated with changes in glycerophospholipid, nucleotide, and amino acid metabolism, with reductions in cardiac glucose catabolism. Collectively, these results suggest that substantial metabolic changes occur in the maternal heart during and after pregnancy.