Brassica napus genes Rlm4 and Rlm7, conferring resistance to Leptosphaeria maculans, are alleles of the Rlm9 wall‐associated kinase‐like resistance locus

To prove the function of the predicted genes, the entire gene including introns and 5’ intergenic region (1750 bp) for each allele was synthesized (GenScript, USA) and cloned into the plant transformation vector pMDC123, modified to contain the nosT terminator sequence downstream of the cloning site...

Full description

Saved in:
Bibliographic Details
Published in:Plant biotechnology journal 2022-07, Vol.20 (7), p.1229-1231
Main Authors: Haddadi, Parham, Larkan, Nicholas J., deWouw, Angela, Zhang, Yueqi, Xiang Neik, Ting, Beynon, Elena, Bayer, Philipp, Edwards, Dave, Batley, Jacqueline, Borhan, Mohammad Hossein
Format: Article
Language:English
Subjects:
Alleles
Amino acids
AvrLm4‐7
Binding
blackleg
Brassica
Brief Communication
Brief Communications
Calcium ions
Canola
Cloning
Conflicts of interest
disease resistance gene
Drug resistance in microorganisms
Expression vectors
Fibroblast growth factor 2
Genes
Genetic aspects
Genetic transformation
Genomes
Growth factors
Infections
Introns
Kinases
Membrane proteins
Pathogens
Peptides
Polymorphism
Protein kinase
Protein structure
Protein-serine/threonine kinase
Proteins
Rape plants
transmembrane receptors
Virulence
Virulence (Microbiology)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To prove the function of the predicted genes, the entire gene including introns and 5’ intergenic region (1750 bp) for each allele was synthesized (GenScript, USA) and cloned into the plant transformation vector pMDC123, modified to contain the nosT terminator sequence downstream of the cloning site using Gateway cloning technology. Protein structure is represented by the signal peptide (yellow bar), extracellular GUB_WAK (dark blue), C-terminal WAK (blue bar), and EGF-like Ca2+ (grey bar), transmembrane (red bar) and an intracellular serine/threonine Protein Kinase (green bar) domains. (e, f) Expression profile of Rlm4, Rlm7, and AvrLm4-7. InterPro predicts Rlm4 and Rlm7 as transmembrane proteins consisting of secretory signal peptide (SP), extracellular wall-associated receptor kinase galacturonan- binding (WAK_GUB), WAK, epithelial growth factor (EGF) like calcium binding domains and a cytoplasmic kinase domain (Figure 1d).
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.13818