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Lactococcus lactis subsp. cremoris C60 induces macrophages activation that enhances CD4+ T cell-based adaptive immunity
Lactococcus lactis subsp. cremoris C60 is a probiotic strain that induces diverse functional modifications in immune cells. In this report, as a novel effect of C60 on myeloid lineage cells, we show that C60 enhances the immunological function of macrophages that consequently promotes CD4+ T cell ac...
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Published in: | Bioscience of Microbiota, Food and Health Food and Health, 2022, Vol.41(3), pp.130-136 |
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description | Lactococcus lactis subsp. cremoris C60 is a probiotic strain that induces diverse functional modifications in immune cells. In this report, as a novel effect of C60 on myeloid lineage cells, we show that C60 enhances the immunological function of macrophages that consequently promotes CD4+ T cell activity in an antigen-dependent manner. Heat-killed (HK) C60 induced the production of pro-inflammatory cytokines in thioglycolate-elicited peritoneal macrophages (TPMs) much stronger than Toll-like receptor (TLR) ligand stimulation. The HK-C60 treatment also augmented the expression of antigen-presenting and co-stimulatory molecules, such as major histocompatibility complex (MHC) class II, CD80, and CD86, as well as antigen uptake in TPMs. These HK-C60-mediated functional upregulations in TPMs resulted in the promotion of CD4+ T cell activation in an antigen-dependent manner. Interestingly, the TPMs that originated from the mice fed the HK-C60 diet showed pre-activated characteristics, which was confirmed by the upregulation of cytokine production and antigen presentation-related molecule expression under lipopolysaccharide (LPS) stimulation. Furthermore, the antigen-dependent CD4+ T cell activation was also enhanced by the TPMs. This implied that antigen presentation activity was enhanced in the TPMs that originated from the HK-C60 diet mice. Thus, C60 effectively upregulates the immunological function of macrophages that directly connects to CD4+ T cell-based adaptive immunity. |
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In this report, as a novel effect of C60 on myeloid lineage cells, we show that C60 enhances the immunological function of macrophages that consequently promotes CD4+ T cell activity in an antigen-dependent manner. Heat-killed (HK) C60 induced the production of pro-inflammatory cytokines in thioglycolate-elicited peritoneal macrophages (TPMs) much stronger than Toll-like receptor (TLR) ligand stimulation. The HK-C60 treatment also augmented the expression of antigen-presenting and co-stimulatory molecules, such as major histocompatibility complex (MHC) class II, CD80, and CD86, as well as antigen uptake in TPMs. These HK-C60-mediated functional upregulations in TPMs resulted in the promotion of CD4+ T cell activation in an antigen-dependent manner. Interestingly, the TPMs that originated from the mice fed the HK-C60 diet showed pre-activated characteristics, which was confirmed by the upregulation of cytokine production and antigen presentation-related molecule expression under lipopolysaccharide (LPS) stimulation. Furthermore, the antigen-dependent CD4+ T cell activation was also enhanced by the TPMs. This implied that antigen presentation activity was enhanced in the TPMs that originated from the HK-C60 diet mice. Thus, C60 effectively upregulates the immunological function of macrophages that directly connects to CD4+ T cell-based adaptive immunity.</description><identifier>ISSN: 2186-3342</identifier><identifier>ISSN: 2186-6953</identifier><identifier>EISSN: 2186-3342</identifier><identifier>DOI: 10.12938/bmfh.2021-057</identifier><identifier>PMID: 35854694</identifier><language>eng</language><publisher>Tokyo: BMFH Press</publisher><subject>Adaptive immunity ; Antigen presentation ; Antigens ; CD4 antigen ; CD4+ T cells ; CD80 antigen ; CD86 antigen ; Cell activation ; Cytokines ; Diet ; IFN-γ ; IL-12/IL-23p40 ; IL-6 ; Immune system ; Immunity ; Immunology ; Inflammation ; lactic acid bacteria ; Lactococcus lactis ; Lipopolysaccharides ; Lymphocytes ; Lymphocytes T ; Macrophages ; Major histocompatibility complex ; Probiotics ; Stimulation ; TNF-α ; Toll-like receptors</subject><ispartof>Bioscience of Microbiota, Food and Health, 2022, Vol.41(3), pp.130-136</ispartof><rights>2022 by BMFH Press</rights><rights>2022. 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In this report, as a novel effect of C60 on myeloid lineage cells, we show that C60 enhances the immunological function of macrophages that consequently promotes CD4+ T cell activity in an antigen-dependent manner. Heat-killed (HK) C60 induced the production of pro-inflammatory cytokines in thioglycolate-elicited peritoneal macrophages (TPMs) much stronger than Toll-like receptor (TLR) ligand stimulation. The HK-C60 treatment also augmented the expression of antigen-presenting and co-stimulatory molecules, such as major histocompatibility complex (MHC) class II, CD80, and CD86, as well as antigen uptake in TPMs. These HK-C60-mediated functional upregulations in TPMs resulted in the promotion of CD4+ T cell activation in an antigen-dependent manner. Interestingly, the TPMs that originated from the mice fed the HK-C60 diet showed pre-activated characteristics, which was confirmed by the upregulation of cytokine production and antigen presentation-related molecule expression under lipopolysaccharide (LPS) stimulation. Furthermore, the antigen-dependent CD4+ T cell activation was also enhanced by the TPMs. This implied that antigen presentation activity was enhanced in the TPMs that originated from the HK-C60 diet mice. Thus, C60 effectively upregulates the immunological function of macrophages that directly connects to CD4+ T cell-based adaptive immunity.</description><subject>Adaptive immunity</subject><subject>Antigen presentation</subject><subject>Antigens</subject><subject>CD4 antigen</subject><subject>CD4+ T cells</subject><subject>CD80 antigen</subject><subject>CD86 antigen</subject><subject>Cell activation</subject><subject>Cytokines</subject><subject>Diet</subject><subject>IFN-γ</subject><subject>IL-12/IL-23p40</subject><subject>IL-6</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>lactic acid bacteria</subject><subject>Lactococcus lactis</subject><subject>Lipopolysaccharides</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Major histocompatibility complex</subject><subject>Probiotics</subject><subject>Stimulation</subject><subject>TNF-α</subject><subject>Toll-like receptors</subject><issn>2186-3342</issn><issn>2186-6953</issn><issn>2186-3342</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpVkU1v1DAQhi0EolXplbMljiiLv-NckFD4lFbiUs7WxHEarxIn2E5R_z0OW1bgg_2O55mxNS9Cryk5UNZw_a6bh_HACKMVkfUzdM2oVhXngj3_R1-h25ROpCxFpBL8JbriUkuhGnGNfh3B5sUu1m4JT0X7hNPWpfWAbXTzEkvcKoJ96DfrEp7BxmUd4b7onX6A7JeA8wgZuzBC2KH2o3iL77B101R1kFyPoYe1wA77ed6Cz4-v0IsBpuRun84b9OPzp7v2a3X8_uVb--FYWSlkrnrtoBeN6GomNZHSUgrSOjb0IBmvS-h0udVDB0TR3tFOOSolJR3RTc00v0Hvz33XrZtdb13IESazRj9DfDQLePN_JvjR3C8PpmFCCVqXBm-eGsTl5-ZSNqdli6H82TCleSGEVoU6nKkynZSiGy4vUGL-eGV2r8zulSlelYL2XHBKuQzzgkPM3k7uTAtq-L79rbpk7QjRuMB_Aw5Cn3A</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>SAITO, Suguru</creator><creator>OKUNO, Alato</creator><creator>KAKIZAKI, Nanae</creator><creator>MAEKAWA, Toshio</creator><creator>TSUJI, Noriko M.</creator><general>BMFH Press</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20220101</creationdate><title>Lactococcus lactis subsp. cremoris C60 induces macrophages activation that enhances CD4+ T cell-based adaptive immunity</title><author>SAITO, Suguru ; 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In this report, as a novel effect of C60 on myeloid lineage cells, we show that C60 enhances the immunological function of macrophages that consequently promotes CD4+ T cell activity in an antigen-dependent manner. Heat-killed (HK) C60 induced the production of pro-inflammatory cytokines in thioglycolate-elicited peritoneal macrophages (TPMs) much stronger than Toll-like receptor (TLR) ligand stimulation. The HK-C60 treatment also augmented the expression of antigen-presenting and co-stimulatory molecules, such as major histocompatibility complex (MHC) class II, CD80, and CD86, as well as antigen uptake in TPMs. These HK-C60-mediated functional upregulations in TPMs resulted in the promotion of CD4+ T cell activation in an antigen-dependent manner. Interestingly, the TPMs that originated from the mice fed the HK-C60 diet showed pre-activated characteristics, which was confirmed by the upregulation of cytokine production and antigen presentation-related molecule expression under lipopolysaccharide (LPS) stimulation. Furthermore, the antigen-dependent CD4+ T cell activation was also enhanced by the TPMs. This implied that antigen presentation activity was enhanced in the TPMs that originated from the HK-C60 diet mice. Thus, C60 effectively upregulates the immunological function of macrophages that directly connects to CD4+ T cell-based adaptive immunity.</abstract><cop>Tokyo</cop><pub>BMFH Press</pub><pmid>35854694</pmid><doi>10.12938/bmfh.2021-057</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adaptive immunity Antigen presentation Antigens CD4 antigen CD4+ T cells CD80 antigen CD86 antigen Cell activation Cytokines Diet IFN-γ IL-12/IL-23p40 IL-6 Immune system Immunity Immunology Inflammation lactic acid bacteria Lactococcus lactis Lipopolysaccharides Lymphocytes Lymphocytes T Macrophages Major histocompatibility complex Probiotics Stimulation TNF-α Toll-like receptors |
title | Lactococcus lactis subsp. cremoris C60 induces macrophages activation that enhances CD4+ T cell-based adaptive immunity |
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