A potential oral microbiome signature associated with coronary artery disease in Tunisia

• Published in: Bioscience reports 2022-07, Vol.42 (7), p.1
• Main Authors: Bouzid, Fériel, Gtif, Imen, Alfadhli, Suad, Charfeddine, Salma, Ghorbel, Walid, Abdelhédi, Rania, Benmarzoug, Riadh, Abid, Leila, Bouayed Abdelmoula, Nouha, Elloumi, Inés, Masmoudi, Saber, Rebai, Ahmed, Kharrat, Najla
• Format: Article
• Language: English
• Subjects: Abundance; Arteriosclerosis; Atherosclerosis; Bacteria; Bacteria - genetics; Biotechnology; Cardiovascular disease; Cerebral infarction; Coronary artery disease; Coronary Artery Disease - genetics; Coronary vessels; Eikenella; Environmental factors; Gene sequencing; Genomics; Gum disease; Heart diseases; Humans; Inflammatory diseases; Metagenome; Metagenomics; Microbial activity; Microbiology; Microbiomes; Microbiota; Microbiota - genetics; Microorganisms; Myocardial infarction; Neisseria; Next-generation sequencing; Regression analysis; Relative abundance; RNA, Ribosomal, 16S - genetics; rRNA 16S; Streptococcus; Streptococcus infections; Syntax; Taxonomy; Tunisia - epidemiology; Vein & artery diseases; Womens health
• ISSN: 0144-8463; 1573-4935; 1573-4935
• DOI: 10.1042/BSR20220583

The coronary artery disease (CAD) is a chronic inflammatory disease involving genetic as well as environmental factors. Recent evidence suggests that the oral microbiome has a significant role in triggering atherosclerosis. The present study assessed the oral microbiome composition variation between coronary patients and healthy subjects in order to identify a potential pathogenic signature associated with CAD. We performed metagenomic profiling of salivary microbiomes by 16S ribosomal RNA (rRNA) next-generation sequencing. Oral microbiota profiling was performed for 30 individuals including 20 patients with CAD and ten healthy individuals without carotid plaques or previous stroke or myocardial infarction. We found that oral microbial communities in patients and healthy controls are represented by similar global core oral microbiome. The predominant taxa belonged to Firmicutes (genus Streptococcus, Veillonella, Granulicatella, Selenomonas), Proteobacteria (genus Neisseria, Haemophilus), Actinobacteria (genus Rothia), Bacteroidetes (genus Prevotella, Porphyromonas), and Fusobacteria (genus Fusobacterium, Leptotrichia). More than 60% relative abundance of each sample for both CAD patients and controls is represented by three major genera including Streptococcus (24.97 and 26.33%), Veillonella (21.43 and 19.91%), and Neisseria (14.23 and 15.33%). Using penalized regression analysis, the bacterial genus Eikenella was involved as the major discriminant genus for both status and Syntax score of CAD. We also reported a significant negative correlation between Syntax score and Eikenella abundance in coronary patients' group (Spearman rho = -0.68, P=0.00094). In conclusion, the abundance of Eikenella in oral coronary patient samples compared with controls could be a prominent pathological indicator for the development of CAD.