Preventative effects of antioxidants on changes in sebocytes, outer root sheath cells, and Cutibacterium acnes-pretreated mice by particulate matter: No significant difference among antioxidants

• Published in: International journal of immunopathology and pharmacology 2022-06, Vol.36, p.3946320221112433
• Main Authors: Kwack, Mi Hee, Ha, Dae-Lyong, Lee, Weon Ju
• Format: Article
• Language: English
• Subjects: Acne Vulgaris - metabolism; Acne Vulgaris - microbiology; Animals; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Biomarkers; Cell viability; Cutibacterium acnes; Cytokines; Cytokines - metabolism; Epigallocatechin gallate; Inflammation; Matrix metalloproteinase; Metalloproteinase; Mice; NF-κB protein; Original; Particulate matter; Particulate Matter - metabolism; Particulate Matter - toxicity; Propionibacterium acnes - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Resveratrol; Resveratrol - pharmacology; Sebaceous Glands - metabolism; Sebaceous Glands - microbiology
• ISSN: 0394-6320; 2058-7384; 2058-7384
• DOI: 10.1177/03946320221112433

Objectives Particulate matter (PM) is an air pollutant that can damage human skin; antioxidants have shown some efficacy in alleviating PM-induced skin inflammation. We investigated the antioxidant effects of punicalagin, epigallocatechin-3-gallate (EGCG), and resveratrol on PM-induced changes in cultured human sebocytes, outer root sheath (ORS) cells, and Cutibacterium acnes-pretreated mice. Methods Sebocytes and ORS cells were cultured with 100 μg/mL PM10 and 5 μM punicalagin, 1 μM EGCG, or 1 μM resveratrol for 24 h. In C. acnes-pretreated mice, inflammatory nodules were treated with 100 μg/mL PM10 and 5 μM punicalagin, 1 μM EGCG, or 1 μM resveratrol. Cell viability was measured using an MTT assay. Antioxidant effects were analyzed according to RNA expression, using real-time PCR, as well as reactive oxygen species (ROS) and sebum measurements. Results Antioxidants inhibited the upregulation of inflammatory cytokines, matrix metalloproteinase, aryl hydrocarbon receptor, and NF-kB as well as the production of ROS induced by PM10 in cultured sebocytes and ORS cells. The preventative effects of punicalagin and EGCG on biomarker expression in cultured sebocytes and ORS cells were slightly greater than those of resveratrol, though the difference was not significant. In C. acnes-pretreated mice, the antioxidants inhibited inflammatory cytokine and matrix metalloproteinase expression as well as sebum production. Conclusions Antioxidants effectively reduced the expression of inflammatory biomarkers and sebum production in cultured sebocytes, ORS cells, and C. acnes-pretreated mice.