SDF‐1‐edited human amniotic mesenchymal stem cells stimulate angiogenesis in treating hindlimb ischaemia

Although stem cells have extensively been studied as a novel vehicle for tissue repair, their sustained efficacy remains controversial. In this study, we aimed to investigate the angiogenic potency over time of stromal cell‐derived factor‐1 (SDF‐1) gene‐edited amniotic mesenchymal stem cells (AMM/S)...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2022-07, Vol.26 (13), p.3726-3735
Main Authors: Zhang, Hong Zhe, Han, Seongho, Kim, Sung‐Whan
Format: Article
Language:English
Subjects:
Angiogenesis
Animal models
Cell culture
Cell migration
cell therapy
Fibroblasts
Genome editing
Genomes
Growth factors
ischaemia
Ischemia
Laboratory animals
Mesenchymal stem cells
Microscopy
Microvasculature
Nuclease
Original
Perfusion
Polymerase chain reaction
SDF‐1
Stem cell transplantation
Stem cells
Transcription activator-like effector nucleases
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Summary:Although stem cells have extensively been studied as a novel vehicle for tissue repair, their sustained efficacy remains controversial. In this study, we aimed to investigate the angiogenic potency over time of stromal cell‐derived factor‐1 (SDF‐1) gene‐edited amniotic mesenchymal stem cells (AMM/S) in a hindlimb ischaemia model. An SDF‐1 transgene was inserted into the AMM cell genome via transcription activator‐like effector nuclease (TALEN) mediated knock‐in, and cell migration, Matrigel tube formation, and in vivo Matrigel plug assays were performed. AMM/S were also transplanted into hindlimb ischaemia model mice. Blood perfusion, therapeutic potential, histology, capillary density and in vivo angiogenic assays were performed. AMM/S exhibited high expression of the SDF‐1 gene, and robustly promoted migration, proliferation and microvascular formation. AMM/S transplantation significantly increased blood perfusion and limb loss prevention compared with AMM. AMM/S also significantly inhibited increased capillary density and expression of angiogenic factors in the ischaemic hindlimb. Our study demonstrated that AMM/S provides a significant therapeutic effect in ischaemic hindlimbs by enhancing angiogenesis.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.17401