Evaluation of PCR-Generated Chimeras, Mutations, and Heteroduplexes with 16S rRNA Gene-Based Cloning

• Published in: Applied and Environmental Microbiology 2001-02, Vol.67 (2), p.880-887
• Main Authors: Qiu, X, Wu, L, Huang, H, McDonel, P E, Palumbo, A V, Tiedje, J M, Zhou, J
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Cloning; Cloning, Molecular - methods; Deoxyribonuclease I - metabolism; DNA-Directed DNA Polymerase - metabolism; Ecosystem; Fundamental and applied biological sciences. Psychology; Genes; Genes, rRNA - genetics; Gram-Positive Bacteria - genetics; Methods; Microbiology; Mutation; Mutation - genetics; Nucleic Acid Heteroduplexes - genetics; Nucleic Acid Heteroduplexes - metabolism; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Proteobacteria; Proteobacteria - genetics; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Ribonucleic acid; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Taq DNA polymerase
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.67.2.880-887.2001

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