Lack of TRPV1 Channel Modulates Mouse Gene Expression and Liver Proteome with Glucose Metabolism Changes

To investigate the role of the transient receptor potential channel vanilloid type 1 (TRPV1) in hepatic glucose metabolism, we analyzed genes related to the clock system and glucose/lipid metabolism and performed glycogen measurements at ZT8 and ZT20 in the liver of C57Bl/6J (WT) and Trpv1 KO mice....

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Bibliographic Details
Published in:International journal of molecular sciences 2022-06, Vol.23 (13), p.7014
Main Authors: Lacerda, José Thalles, Gomes, Patrícia R. L., Zanetti, Giovanna, Mezzalira, Nathana, Lima, Otoniel G., de Assis, Leonardo V. M., Guler, Ali, Castrucci, Ana Maria, Moraes, Maria Nathália
Format: Article
Language:English
Subjects:
AKT protein
Body fat
Capsaicin receptors
Circadian rhythm
Clock systems
Energy
Enzymes
Gene expression
Genotype & phenotype
Glucagon
Gluconeogenesis
Glucose
Glucose metabolism
Glucose transporter
Glycogen
Glycogens
Homeostasis
Inflammation
Kinases
Lipid metabolism
Lipids
Liver
Metabolism
Period 1 protein
Peroxisome proliferator-activated receptors
Physiology
Protein kinase A
Proteomes
Proteomics
Rodents
Signal transduction
Transient receptor potential proteins
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Summary:To investigate the role of the transient receptor potential channel vanilloid type 1 (TRPV1) in hepatic glucose metabolism, we analyzed genes related to the clock system and glucose/lipid metabolism and performed glycogen measurements at ZT8 and ZT20 in the liver of C57Bl/6J (WT) and Trpv1 KO mice. To identify molecular clues associated with metabolic changes, we performed proteomics analysis at ZT8. Liver from Trpv1 KO mice exhibited reduced Per1 expression and increased Pparα, Pparγ, Glut2, G6pc1 (G6pase), Pck1 (Pepck), Akt, and Gsk3b expression at ZT8. Liver from Trpv1 KO mice also showed reduced glycogen storage at ZT8 but not at ZT20 and significant proteomics changes consistent with enhanced glycogenolysis, as well as increased gluconeogenesis and inflammatory features. The network propagation approach evidenced that the TRPV1 channel is an intrinsic component of the glucagon signaling pathway, and its loss seems to be associated with increased gluconeogenesis through PKA signaling. In this sense, the differentially identified kinases and phosphatases in WT and Trpv1 KO liver proteomes show that the PP2A phosphatase complex and PKA may be major players in glycogenolysis in Trpv1 KO mice.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23137014