Efficacy of bi-valent whole cell inactivated bacterial vaccine against Motile Aeromonas Septicemia (MAS) in cultured catfishes (Heteropneustes fossilis, Clarias batrachus and pangasius pangasius) in Bangladesh

• Published in: Saudi journal of biological sciences 2022-05, Vol.29 (5), p.3881-3889
• Main Authors: Rahman, Mohummad Muklesur, Rahman, Md. Ashikur, Hossain, Muhammad Tofazzal, Siddique, Mahbubul Pratik, Haque, Md. Enamul, Khasruzzaman, A.K.M., Islam, Md. Alimul
• Format: Article
• Language: English
• Subjects: Aeromonas spp; Bi-valent vaccine; IgM; Motile Aeromonad Septicemia (MAS); Original; RPS; Shing, Magur and Pangas
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2022.03.012

The Motile Aeromonas Septicemia (MAS) is an important disease of cultured catfishes (Heteropneustes fossilis, Clarias batrachus and Pangasius pangasius), caused by different species of Aeromonas bacteria which have been documented to be higher death rates (≤70%) in Bangladesh since 2016. Present study was conducted to develop bi-valent vaccine using A. hydrophila and A. veronii, and to validate their efficacy via intra-muscular (IM) and oral-routes of immunization in selected species of fishes. Brood fishes of the three species were immunized with three doses of inactivated vaccine (107 CFU /2.3 mg/ml). Hematological parameters of brood fishes and antibody levels (IgM) of broods, their larvae and eggs were determined by ELISA. Additionally, Relative Percent Survivability (RPS) and the IgM levels of the larvae after challenge with virulent A. hydrophila and A. veronii were also evaluated. Findings of this study showed that the lymphocytes, monocytes, granulocytes counts and antibody (IgM) titre of brood fishes, larvae and eggs from the vaccinated fishes were found significantly higher (p