DLC-1 facilitates germ granule assembly in Caenorhabditis elegans embryo

Germ granules are cytoplasmic assemblies of RNA-binding proteins (RBPs) required for germ cell development and fertility. During the first four cell divisions of the zygote, regulated assembly of germ (P) granules leads to their selective segregation to the future germ cell. Here we investigate the...

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Bibliographic Details
Published in:Molecular biology of the cell 2022-05, Vol.33 (5), p.ar41-ar41
Main Authors: Day, Nicholas J, Ellenbecker, Mary, Wang, Xiaobo, Voronina, Ekaterina
Format: Article
Language:English
Subjects:
Animals
Caenorhabditis elegans - metabolism
Caenorhabditis elegans Proteins - metabolism
Cytoplasmic Granules - metabolism
Dyneins - metabolism
Germ Cell Ribonucleoprotein Granules
Germ Cells - metabolism
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Summary:Germ granules are cytoplasmic assemblies of RNA-binding proteins (RBPs) required for germ cell development and fertility. During the first four cell divisions of the zygote, regulated assembly of germ (P) granules leads to their selective segregation to the future germ cell. Here we investigate the role of DLC-1, a hub protein implicated in stabilization and function of diverse protein complexes, in maintaining P granule integrity. We find that DLC-1 directly interacts with several core P granule proteins, predominantly during embryogenesis. The loss of disrupts assembly of P granule components into phase-separated organelles in the embryos, regardless of whether or not DLC-1 directly interacts with these proteins. Finally, we infer that P granule dispersal in the absence of is likely independent of DLC-1's function as a subunit of the dynein motor and does not result from a loss of cell polarity.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E21-05-0275