Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis

Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-indu...

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Bibliographic Details
Published in:Yàowu shi͡p︡in fenxi 2019-04, Vol.27 (2), p.518-525
Main Authors: Guo, Ling, Jiang, Xiao, Tian, Hao-Yuan, Yao, Shang-Jin, Li, Bo-Ya, Zhang, Rong-Jie, Zhang, Shu-Sheng, Sun, Xin
Format: Article
Language:English
Subjects:
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide - chemistry
Adducts
Aromatic compounds
Aromatic hydrocarbons
Benzo(a)pyrene-diol-epoxide
Birth
Birth defects
Blood
BPDE-dG
Carcinogenesis
Carcinogenicity
Carcinogens
Chromatography, Liquid
Congenital defects
Cord blood
Deoxyribonucleic acid
DNA
DNA adducts
DNA Adducts - blood
DNA Adducts - chemistry
Epidemiology
Female
Fetal Blood - chemistry
Fetuses
Human biomonitoring
Humans
LC-MS/MS
Metabolism
Molecular Conformation
Molecular epidemiology
Mutagenicity
Original
Phosphatase
Polycyclic aromatic hydrocarbons
Pregnancy
Pyrene
Scientific imaging
Studies
Tandem Mass Spectrometry
Teratogenicity
Umbilical cord
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Summary:Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups. [Display omitted]
ISSN:1021-9498
2224-6614
DOI:10.1016/j.jfda.2019.03.001