Intergeneric hybridization of marguerite (Argyranthemum frutescens (L.) Sch. Bip.) and Roman chamomile (Chamaemelum nobile (L.) All.) using ovule culture and confirmation of hybridity

To introduce useful characteristics such as fragrance into Argyranthemum frutescens (L.) and to expand the variation, we conducted crosses using A. frutescens as the seed parent and Chamaemelum nobile (L.) All. as the pollen parent. All the tested cross combinations between the three strains of A. f...

Full description

Saved in:
Bibliographic Details
Published in:Plant Biotechnology 2022/06/25, Vol.39(2), pp.93-100
Main Authors: Katsuoka, Hiroyuki, Hamabe, Naoya, Kato, Chiemi, Hisamatsu, Susumu, Baba, Fujio, Taneishi, Motohiro, Sasaki, Toshiyuki, Ikegaya, Atsushi, Inaba, Zentaro
Format: Article
Language:English
Subjects:
Amplification
Argyranthemum
Argyranthemum frutescens
CAPS marker
Chamaemelum
Chamaemelum nobile
Cross-breeding
Embryos
Flow cytometry
Hybrids
Intergeneric hybridization
Markers
Original Paper
Plantlets
Pollen
Sequence analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To introduce useful characteristics such as fragrance into Argyranthemum frutescens (L.) and to expand the variation, we conducted crosses using A. frutescens as the seed parent and Chamaemelum nobile (L.) All. as the pollen parent. All the tested cross combinations between the three strains of A. frutescens and one strain of C. nobile produced embryos, and healthy plants were obtained by ovule culture. The obtained plantlets had a white ray floret, and the leaf shape was intermediate to those of the parents. The individuals obtained from this cross were subjected to two methods to determine hybridity: flow cytometry analyses and cleaved amplified polymorphic sequence (CAPS) markers. For the CAPS marker, we selected the internal transcribed spacer (ITS) region, which is highly variable among the genera, as the region to be amplified. We selected restriction enzymes BmgT120 I and Afl II, which selectively cut common sequences in the genus Argyranthemum, based on the sequence analysis of one parent strain each of A. frutescens and C. nobile and alignment with known sequences of related species. Flow cytometry analyses and CAPS markers revealed that the individuals obtained from the cross between A. frutescens and C. nobile are intergeneric hybrids. In addition, these established methods were capable of quickly and reliably identifying hybrids between A. frutescens and C. nobile. This report shows for the first time that crossbreeding between A. frutescens (seed parent) and C. nobile (pollen parent) is possible, and further development of Argyranthemum breeding, such as the expansion of variation by intergeneric crosses, is expected.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.21.1130a