Metataxonomic and metabolomic evidence of biofilm homeostasis disruption related to caries: An in vitro study

The ecological dysbiosis of a biofilm includes not only bacterial changes but also changes in their metabolism. Related to oral biofilms, changes in metabolic activity are crucial endpoint, linked directly to the pathogenicity of oral diseases. Despite the advances in caries research, detailed micro...

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Bibliographic Details
Published in:Molecular oral microbiology 2022-04, Vol.37 (2), p.81-96
Main Authors: Sánchez, María C., Velapatiño, Angela, Llama‐Palacios, Arancha, Valdés, Alberto, Cifuentes, Alejandro, Ciudad, María J., Collado, Luis
Format: Article
Language:English
Subjects:
Abundance
Amino acids
Biofilms
caries
Dental caries
Dental Caries - microbiology
Dental Caries Susceptibility
Dysbacteriosis
Etiology
Gene sequencing
Glutamic acid
High performance liquid chromatography
Histidine
Homeostasis
Humans
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Metabolomics
metataxonomics
Methionine
Microbiota
Microorganisms
Original
Pathogenicity
Pathogens
Peptides
Phenylalanine
Protein metabolism
Protein turnover
Quadrupoles
Relative abundance
RNA, Ribosomal, 16S - genetics
rRNA 16S
Saliva
Streptococcus
Sucrose
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Summary:The ecological dysbiosis of a biofilm includes not only bacterial changes but also changes in their metabolism. Related to oral biofilms, changes in metabolic activity are crucial endpoint, linked directly to the pathogenicity of oral diseases. Despite the advances in caries research, detailed microbial and metabolomic etiology is yet to be fully clarified. To advance this knowledge, a meta‐taxonomic approach based on 16S rRNA gene sequencing and an untargeted metabolomic approach based on an ultra‐high performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry analysis (UHPLC/Q‐TOF‐MS) were conducted. To this end, an in vitro biofilm model derived from the saliva of healthy participants were developed, under commensal and cariogenic conditions by adding sucrose as the disease trigger. The cariogenic biofilms showed a significant increase of Firmicutes phyla (p = 0.019), due to the significant increase in the genus Streptococcus (p = 0.010), and Fusobacter (p 
ISSN:2041-1006
2041-1014
DOI:10.1111/omi.12363